Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605210748_cabf GAAGGAGGGTCAAGGAAAATCGAGGGAAGATG(+CAGTGT)GTACCAAGGCACAGCACAGGCCCACCCAGGC NGG 10 8 17 8 24 0 100 5 10
    240605210747_o7i3 CATGCATATTATTTATTTCTGTGCAAACTGTG(+CAGTGT)GATCCGTAACCAAGGTGAAGAAAGTTGGGAT NGG 10 8 17 8 24 0 100 5 10
    240605210745_g7nz CAGAAATCCCAACTTTCTTCACCTTGGTTACG(+CAGTGT)GATCCACAGTTTGCACAGAAATAAATAATAT NGG 10 8 17 8 24 0 100 5 10
    240605210743_uger CTTCACATAGCCACATCATAGACGTTTTTAGG(+CAGTGT)GATCCCTGCTCTCCTAAATTGCTCCCCGAGT NGG 10 8 17 8 24 0 100 5 10
    240605210741_cwd4 TACTGACTCGGGGAGCAATTTAGGAGAGCAGG(+CAGTGT)GATCCCTAAAAACGTCTATGATGTGGCTATG NGG 10 8 17 8 24 0 100 5 10
    240605210739_153p CCTAACTCTGAGCAAGTGACTTCTGCAAAAGG(+CAGTGT)GTACCCCGAAGGAAGCCATGTGGGCATCCTG NGG 10 8 17 8 24 0 100 5 10
    240605210737_ib6y CTGGACAGGATGCCCACATGGCTTCCTTCGGG(+CAGTGT)GTACCCTTTTGCAGAAGTCACTTGCTCAGAG NGG 10 8 17 8 24 0 100 5 10
    240605210736_nr16 TGGTCTGGACAGAGGTGTGCCCCATCCAGACG(+CAGTGT)GTACCAGGATAGCAGCAGCTGCATGGCGCTA NGG 10 8 17 8 24 0 100 5 10
    240605210733_bovz ACAGGTAGCGCCATGCAGCTGCTGCTATCCTG(+CAGTGT)GTACCGTCTGGATGGGGCACACCTCTGTCCA NGG 10 8 17 8 24 0 100 5 10
    240605210731_ho4c CTGCCCACTGGCCGTGCGCAGGGAGAACCTGG(+CAGTGT)GATCCCATTCATCGGAGTGACACATCACGAT NGG 10 8 17 8 24 0 100 5 10