Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605212949_vzcw TCTTTGAGCTAATGGTGATGTGGTATGCAGAG(+CAGTGT)GTACCAGGGGCCATGGGGGTGTGTGTGCTTC NGG 10 8 17 8 24 0 100 5 10
    240605212945_95be GACAGGAAGCACACACACCCCCATGGCCCCTG(+CAGTGT)GTACCTCTGCATACCACATCACCATTAGCTC NGG 10 8 17 8 24 0 100 5 10
    240605212943_revb ACCCTAGAAGGTAGGGACAAGTGAAGGACTGG(+CAGTGT)GATCCTTTTTGCAGTATGTTCCTTGACTCGC NGG 10 8 17 8 24 0 100 5 10
    240605212942_7khu ATGTGGCGAGTCAAGGAACATACTGCAAAAAG(+CAGTGT)GATCCCAGTCCTTCACTTGTCCCTACCTTCT NGG 10 8 17 8 24 0 100 5 10
    240605212940_yrgs GTAGTGAGATGTAGTATTTACTCTTAACATAG(+CAGTGT)GATCCTGTGGAACAGGTGTTCTGAGAAGACT NGG 10 8 17 8 24 0 100 5 10
    240605212938_31mb AATTCAGTCTTCTCAGAACACCTGTTCCACAG(+CAGTGT)GATCCTATGTTAAGAGTAAATACTACATCTC NGG 10 8 17 8 24 0 100 5 10
    240605212936_nq40 TTAGTCTGTAGCTAATTTCTAAGAAAAACAAG(+CAGTGT)GTACCTTCAATTAAAATTAGAGCTTAGAATA NGG 10 8 17 8 24 0 100 5 10
    240605212934_2elo TTTTATATTCTAAGCTCTAATTTTAATTGAAG(+CAGTGT)GTACCTTGTTTTTCTTAGAAATTAGCTACAG NGG 10 8 17 8 24 0 100 5 10
    240605212933_ixuh TTAACTACAGAACGTAGAAAGCAGTGGAGTGG(+CAGTGT)GTACCGGACCTGTCGCCACACACCCTTACCT NGG 10 8 17 8 24 0 100 5 10
    240605212931_fkx3 TTCTTAGGTAAGGGTGTGTGGCGACAGGTCCG(+CAGTGT)GTACCCACTCCACTGCTTTCTACGTTCTGTA NGG 10 8 17 8 24 0 100 5 10