Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605210854_6dac CCTCTCTTTTCATCCTTTCAGAGACTTTAAGG(+CAGTGT)GTACCGGACCTGCAGCCCAGCTGACCAGACC NGG 10 8 17 8 24 0 100 5 10
    240605210853_qalg CATTAACTAGATATAATGATTTTGCCTTTTTG(+CAGTGT)GTACCTAGTGAAATCTAGAGTATTTTACATT NGG 10 8 17 8 24 0 100 5 10
    240605210851_gh2a GCTGTAATGTAAAATACTCTAGATTTCACTAG(+CAGTGT)GTACCAAAAAGGCAAAATCATTATATCTAGT NGG 10 8 17 8 24 0 100 5 10
    240605210850_ce2n TACCCAGTTCTATTTCTCCTGATAAGATGAAG(+CAGTGT)GTACCTTGAAATCCTGGGAAGGGCTGACATC NGG 10 8 17 8 24 0 100 5 10
    240605210849_vw9b AAGATGATGTCAGCCCTTCCCAGGATTTCAAG(+CAGTGT)GTACCTTCATCTTATCAGGAGAAATAGAACT NGG 10 8 17 8 24 0 100 5 10
    240605210847_yeqs TTGCTAGAATGGTTCCTAGAACTCAGGAAAAG(+CAGTGT)GTACCTTATAAAACCATCACTGGTTTATTTT NGG 10 8 17 8 24 0 100 5 10
    240605210846_f170 CTTTTAAAATAAACCAGTGATGGTTTTATAAG(+CAGTGT)GTACCTTTTCCTGAGTTCTAGGAACCATTCT NGG 10 8 17 8 24 0 100 5 10
    240605210845_5rre GACTTGTGAAAATCAGCTCTCCTCACCATGTG(+CAGTGT)GATCCTGAAGATTGAATTTACGTCTTTCAGG NGG 10 8 17 8 24 0 100 5 10
    240605210843_ndhr CCAAGCCTGAAAGACGTAAATTCAATCTTCAG(+CAGTGT)GATCCACATGGTGAGGAGAGCTGATTTTCAC NGG 10 8 17 8 24 0 100 5 10
    240605210842_rv3a TAGCCAATGACACGTTTTTGACTCAGCGAATG(+CAGTGT)GTACCAGTCACAGGTCCCATCTTGTAGAGTG NGG 10 8 17 8 24 0 100 5 10