Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605210912_1n8i TGCTTTGACAAGGGTGAAAAAACCTCCCCCTG(+CAGTGT)GTACCTATATGGGCATGTCAGCCATTCTGCA NGG 10 8 17 8 24 0 100 5 10
    240605210910_n78a GCTGCTGCCCCAGTACAAAGCTATGGGCTTCG(+CAGTGT)GTACCTCCACAGGGACCTAAGTCTAAACCCC NGG 10 8 17 8 24 0 100 5 10
    240605210906_qel3 TCTGAGGGGTTTAGACTTAGGTCCCTGTGGAG(+CAGTGT)GTACCGAAGCCCATAGCTTTGTACTGGGGCA NGG 10 8 17 8 24 0 100 5 10
    240605210904_o41q CAATGAAGATGCCCAAGTACAGAGTGAGGCAG(+CAGTGT)GTACCTGCTGGTCCACAATGGCCTTCAGCAT NGG 10 8 17 8 24 0 100 5 10
    240605210903_2ht8 TGGGAATGCTGAAGGCCATTGTGGACCAGCAG(+CAGTGT)GTACCTGCCTCACTCTGTACTTGGGCATCTT NGG 10 8 17 8 24 0 100 5 10
    240605210902_7kmc GCTCATAGCGGTTCTGGCCCTTGCATTTCAAG(+CAGTGT)GATCCCAAGTGCTCGTCCTCTGAAGAGTCAG NGG 10 8 17 8 24 0 100 5 10
    240605210900_tzu9 AGGCTCTGACTCTTCAGAGGACGAGCACTTGG(+CAGTGT)GATCCTTGAAATGCAAGGGCCAGAACCGCTA NGG 10 8 17 8 24 0 100 5 10
    240605210859_yu14 TGGACACCTCACAGATGCAGAGGCCACTCTTG(+CAGTGT)GATCCAGCATGTACTGTAGAACACTTCCCCA NGG 10 8 17 8 24 0 100 5 10
    240605210857_f29b ATAACTGGGGAAGTGTTCTACAGTACATGCTG(+CAGTGT)GATCCAAGAGTGGCCTCTGCATCTGTGAGGT NGG 10 8 17 8 24 0 100 5 10
    240605210855_3bib GCAGGGGTCTGGTCAGCTGGGCTGCAGGTCCG(+CAGTGT)GTACCCTTAAAGTCTCTGAAAGGATGAAAAG NGG 10 8 17 8 24 0 100 5 10