Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605210841_g1ju AAGCCCACTCTACAAGATGGGACCTGTGACTG(+CAGTGT)GTACCATTCGCTGAGTCAAAAACGTGTCATT NGG 10 8 17 8 24 0 100 5 10
    240605210839_vcib GTCAGAGAGGGCCATCTCATCAGCTGGGAAAG(+CAGTGT)GTACCTGCCAGCAAGGCTGATAATGTGCTTG NGG 10 8 17 8 24 0 100 5 10
    240605210838_ozqg GGGATCAAGCACATTATCAGCCTTGCTGGCAG(+CAGTGT)GTACCTTTCCCAGCTGATGAGATGGCCCTCT NGG 10 8 17 8 24 0 100 5 10
    240605210836_vssd AAACATCTCCAAACCCATAAAGAGATCGCCTG(+CAGTGT)GTACCGAAACCAGATGAGAAAAGGGGAGGAG NGG 10 8 17 8 24 0 100 5 10
    240605210835_pchx GCTGCCTCCTCCCCTTTTCTCATCTGGTTTCG(+CAGTGT)GTACCAGGCGATCTCTTTATGGGTTTGGAGA NGG 10 8 17 8 24 0 100 5 10
    240605210832_orbd CCAGCAAGGCTGATAATGTGCTTGATCCCCAG(+CAGTGT)GATCCACAGCGTGGAAGGAAAGAACCCATGT NGG 10 8 17 8 24 0 100 5 10
    240605210830_nk2d TGTAGACATGGGTTCTTTCCTTCCACGCTGTG(+CAGTGT)GATCCTGGGGATCAAGCACATTATCAGCCTT NGG 10 8 17 8 24 0 100 5 10
    240605210828_ghwo TTGGTTTGGATGGAAGATGAGCAGCCTGGTGG(+CAGTGT)GATCCTTTGGAGGGTAAGATGGTGATGTTTT NGG 10 8 17 8 24 0 100 5 10
    240605210826_sukl ACTCTAAAACATCACCATCTTACCCTCCAAAG(+CAGTGT)GATCCCACCAGGCTGCTCATCTTCCATCCAA NGG 10 8 17 8 24 0 100 5 10
    240605210824_l9ed TAAAGTGATGGAGGGCAACGGAGGGTGATGGG(+CAGTGT)GATCCTGAAGCCAGGAGTCAGAAATCTTGGT NGG 10 8 17 8 24 0 100 5 10