Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605210415_twfk GCAGCTAGATACACTGCGTGGAGAGATGTGGG(+CAGTGT)GATCCTAGTGACTCTAGGCAAACATGGGCAT NGG 10 8 17 8 24 0 100 5 10
    240605210413_qrtn GGTTGATTGGCGCTCAAGTCCAGGGTGTGTGG(+CAGTGT)GTACCTGTGGCAAAGTAAGGCAGAAGTTGGA NGG 10 8 17 8 24 0 100 5 10
    240605210411_pcor GTTTATCCAACTTCTGCCTTACTTTGCCACAG(+CAGTGT)GTACCCACACACCCTGGACTTGAGCGCCAAT NGG 10 8 17 8 24 0 100 5 10
    240605210409_88rr TTTGGTGCTCAGTAAAGGCCTGCAGAGCCTCG(+CAGTGT)GATCCCGTTTGTGCTTTGAAGTCCTCACATG NGG 10 8 17 8 24 0 100 5 10
    240605210406_3ezd AATTGCATGTGAGGACTTCAAAGCACAAACGG(+CAGTGT)GATCCGAGGCTCTGCAGGCCTTTACTGAGCA NGG 10 8 17 8 24 0 100 5 10
    240605210404_tv3u ATGTTCCTACCGCGGATGCTTTCAGTGCATAG(+CAGTGT)GATCCTCAAGCAACATGAACCTGAAATAAGC NGG 10 8 17 8 24 0 100 5 10
    240605210403_6g7k ACTGGGCTTATTTCAGGTTCATGTTGCTTGAG(+CAGTGT)GATCCTATGCACTGAAAGCATCCGCGGTAGG NGG 10 8 17 8 24 0 100 5 10
    240605210402_svv6 TCAATAATTTTTATCAGATTCTTCTCTGTAGG(+CAGTGT)GTACCTTTCTCATATGTTCTGATTATTTACA NGG 10 8 17 8 24 0 100 5 10
    240605210400_u5x0 TACAGTGTAAATAATCAGAACATATGAGAAAG(+CAGTGT)GTACCCTACAGAGAAGAATCTGATAAAAATT NGG 10 8 17 8 24 0 100 5 10
    240605210358_d8jt GATCTGTATGTATACCACATAATGCGTGCCAG(+CAGTGT)GTACCCACAGAGGTCATAAGAGGGGTAAGAT NGG 10 8 17 8 24 0 100 5 10