Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605210250_zlj5 GCACATGTGCTCCACAGGGTTCACACGGGAAG(+CAGTGT)GATCCCAGGGCTGCATAAGCACCTGTGATTT NGG 10 8 17 8 24 0 100 5 10
    240605210249_7dll ACTGTCTCCAGACACACCAGAAGAGGGCATCG(+CAGTGT)GATCCCATTACAGAATGTTGTGAGCCACTAT NGG 10 8 17 8 24 0 100 5 10
    240605210248_ro8q GCCACATAGTGGCTCACAACATTCTGTAATGG(+CAGTGT)GATCCGATGCCCTCTTCTGGTGTGTCTGGAG NGG 10 8 17 8 24 0 100 5 10
    240605210246_eo3n CTCCAAACCCAACTTTTTACATAGGTGCTGAG(+CAGTGT)GATCCAAACTCAGGTGTTTGTGCCTGCATAT NGG 10 8 17 8 24 0 100 5 10
    240605210245_xc85 GCTTGATATGCAGGCACAAACACCTGAGTTTG(+CAGTGT)GATCCTCAGCACCTATGTAAAAAGTTGGGTT NGG 10 8 17 8 24 0 100 5 10
    240605210244_fp92 CCACGCCTCCCCACCCCCACCTCCCAGCACTG(+CAGTGT)GTACCTTTACAGTGATACTGAGCTGCGTACT NGG 10 8 17 8 24 0 100 5 10
    240605210243_j9gx CTCTGAGTACGCAGCTCAGTATCACTGTAAAG(+CAGTGT)GTACCAGTGCTGGGAGGTGGGGGTGGGGAGG NGG 10 8 17 8 24 0 100 5 10
    240605210241_3ixm TTCATGTTGGGAATCTGTACTGTTTGAAGCAG(+CAGTGT)GTACCGTGAGAAAGGCAGGGGTCTTCTCACA NGG 10 8 17 8 24 0 100 5 10
    240605210240_6vbs TCAGTTGTGAGAAGACCCCTGCCTTTCTCACG(+CAGTGT)GTACCTGCTTCAAACAGTACAGATTCCCAAC NGG 10 8 17 8 24 0 100 5 10
    240605210237_2fct TCACCCCTACCCTGCAGTCCCATAAATAGGGG(+CAGTGT)GTACCCCGAACCAGCCAAGCTTAGTGGGAGG NGG 10 8 17 8 24 0 100 5 10