Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605210235_ox5d CCAGTCCTCCCACTAAGCTTGGCTGGTTCGGG(+CAGTGT)GTACCCCCTATTTATGGGACTGCAGGGTAGG NGG 10 8 17 8 24 0 100 5 10
    240605210233_00yq AATGAAACATGGTCAGAGAGTAGCTGGGTCTG(+CAGTGT)GATCCCACTTCGTGGCCTGGCCTGCTAGTGA NGG 10 8 17 8 24 0 100 5 10
    240605210232_gbnq AACAGTCACTAGCAGGCCAGGCCACGAAGTGG(+CAGTGT)GATCCAGACCCAGCTACTCTCTGACCATGTT NGG 10 8 17 8 24 0 100 5 10
    240605210230_tlvu AAGAGGGAATTAGGAAATGAGGGAATCCATTG(+CAGTGT)GATCCTGGATTCTAGACTGAGACTCTGTGTG NGG 10 8 17 8 24 0 100 5 10
    240605210228_7kj5 CATACCACACAGAGTCTCAGTCTAGAATCCAG(+CAGTGT)GATCCAATGGATTCCCTCATTTCCTAATTCC NGG 10 8 17 8 24 0 100 5 10
    240605210226_3yy7 CATGAGGGAACCAGGCTAGGTGTTTAGCCTTG(+CAGTGT)GATCCCTGATCAAGTTCCATATCAGCTACCC NGG 10 8 17 8 24 0 100 5 10
    240605210225_ta2g CTTGGGGGTAGCTGATATGGAACTTGATCAGG(+CAGTGT)GATCCAAGGCTAAACACCTAGCCTGGTTCCC NGG 10 8 17 8 24 0 100 5 10
    240605210223_hecr GTAGCTCTCAGTGGGACCTGTGGCTGTCCATG(+CAGTGT)GTACCCATGGCAATTGGCCCAGCCTGTGGAT NGG 10 8 17 8 24 0 100 5 10
    240605210221_2120 CTGACATCCACAGGCTGGGCCAATTGCCATGG(+CAGTGT)GTACCATGGACAGCCACAGGTCCCACTGAGA NGG 10 8 17 8 24 0 100 5 10
    240605210219_ptx2 TGTACGCGCGGCACAAAGGTCTGCACTCAGTG(+CAGTGT)GTACCTGCGGGTTGCCCAGCAGGGTTCACAC NGG 10 8 17 8 24 0 100 5 10