Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605211650_4v8o TCAGATGTATATTTAAATTTTTTTTCATCCTG(+CAGTGT)GATCCCAGGAATATAAAAAGAGTAGATATAT NGG 10 8 17 8 24 0 100 5 10
    240605211649_bduu TCAAGGAAAATTGATTTGAAGTATAGACACTG(+CAGTGT)GATCCCCAATCTCTTAAAAATTGCTTCTATT NGG 10 8 17 8 24 0 100 5 10
    240605211647_0frj TTGTTAATAGAAGCAATTTTTAAGAGATTGGG(+CAGTGT)GATCCAGTGTCTATACTTCAAATCAATTTTC NGG 10 8 17 8 24 0 100 5 10
    240605211646_cuxb AGCAGGTTAAATTATCTTAACATCTTTGAATG(+CAGTGT)GTACCTAAGATTTTTCTGAGCACTGTACTGG NGG 10 8 17 8 24 0 100 5 10
    240605211644_3tlh TGATACCAGTACAGTGCTCAGAAAAATCTTAG(+CAGTGT)GTACCATTCAAAGATGTTAAGATAATTTAAC NGG 10 8 17 8 24 0 100 5 10
    240605211643_6oqw CTATGGGTTAAATGGCCCAGCTCTCTTGCCAG(+CAGTGT)GATCCATTTCATTTCCTATAGCGCAGTTAGC NGG 10 8 17 8 24 0 100 5 10
    240605211641_ow59 GGTGTGCTAACTGCGCTATAGGAAATGAAATG(+CAGTGT)GATCCTGGCAAGAGAGCTGGGCCATTTAACC NGG 10 8 17 8 24 0 100 5 10
    240605211640_uhev AAATCCAGAGGGCAAAAGTGTCTGATTAAGTG(+CAGTGT)GATCCTGCAAGGCAGCACCCATAAGTGAACC NGG 10 8 17 8 24 0 100 5 10
    240605211638_9ju6 TCCTGGGTTCACTTATGGGTGCTGCCTTGCAG(+CAGTGT)GATCCACTTAATCAGACACTTTTGCCCTCTG NGG 10 8 17 8 24 0 100 5 10
    240605211636_vrlb CCCCAAGATCTTTGAGATGTCAGAGCTGTGGG(+CAGTGT)GTACCTGCTCAGGAAAACCAGCCAAAGAGAA NGG 10 8 17 8 24 0 100 5 10