Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605205752_0pw5 GGAACGCCTCGATGCTGCTACAGGGATCCCGG(+CAGTGT)GTACCCAGGGAGGACTGCTTATATACACCCT NGG 10 8 17 8 24 0 100 5 10
    240605205749_d7qu GTGCTGGGAGCCCGCCAGGGCTGCTAAACCAG(+CAGTGT)GTACCTCTCTCTGTCTGGAGGTTTGGAAAGA NGG 10 8 17 8 24 0 100 5 10
    240605205745_m6ev CAACTTCTTTCCAAACCTCCAGACAGAGAGAG(+CAGTGT)GTACCTGGTTTAGCAGCCCTGGCGGGCTCCC NGG 10 8 17 8 24 0 100 5 10
    240605205743_b1xi AGTTCAGATGGGAAATTTAGGAGAGTTTTTGG(+CAGTGT)GTACCCGAGTTACTTCACATTATGTAGTACC NGG 10 8 17 8 24 0 100 5 10
    240605205741_4k0q TTTGAGGTACTACATAATGTGAAGTAACTCGG(+CAGTGT)GTACCCAAAAACTCTCCTAAATTTCCCATCT NGG 10 8 17 8 24 0 100 5 10
    240605205739_mfbe TTTTTCCACAGGATAAAGAACATCCAATTTGG(+CAGTGT)GTACCAGAAAGGCTGACATGAAAATTGCTTA NGG 10 8 17 8 24 0 100 5 10
    240605205738_lcmi TAAGATAAGCAATTTTCATGTCAGCCTTTCTG(+CAGTGT)GTACCCAAATTGGATGTTCTTTATCCTGTGG NGG 10 8 17 8 24 0 100 5 10
    240605205735_eutp GTGTATATAAGCAGTCCTCCCTGGGTACCCGG(+CAGTGT)GATCCCTGTAGCAGCATCGAGGCGTTCCTGC NGG 10 8 17 8 24 0 100 5 10
    240605205733_rkkq TTATTGCAGGAACGCCTCGATGCTGCTACAGG(+CAGTGT)GATCCCGGGTACCCAGGGAGGACTGCTTATA NGG 10 8 17 8 24 0 100 5 10
    240605205732_iqug CCATCCATGCTGGGTGCCATCCCACCTCAATG(+CAGTGT)GTACCAATATAACCCCCAACAGACATTTATA NGG 10 8 17 8 24 0 100 5 10