Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605205655_vuzc GCCCCCTGGAACGCAGCTTGATCAACCCCAGG(+CAGTGT)GATCCAGTAGCCCAAACCTGCGCAGACCCCT NGG 10 8 17 8 24 0 100 5 10
    240605205654_g21i ATACAGTGCCTTCACCATGGTCACAAATTCTG(+CAGTGT)GTACCTTTGTGCAAAAGTTGTGGTTGGTTTT NGG 10 8 17 8 24 0 100 5 10
    240605205652_mb7k AGCAAAAAACCAACCACAACTTTTGCACAAAG(+CAGTGT)GTACCAGAATTTGTGACCATGGTGAAGGCAC NGG 10 8 17 8 24 0 100 5 10
    240605205651_vgwx CCTGGCTGGAGATGCTGCTGGGAGAGGCTGTG(+CAGTGT)GTACCCGTAGGGAGAATCCCTAGCTCCCACC NGG 10 8 17 8 24 0 100 5 10
    240605205649_2ygj GTGGAGGTGGGAGCTAGGGATTCTCCCTACGG(+CAGTGT)GTACCACAGCCTCTCCCAGCAGCATCTCCAG NGG 10 8 17 8 24 0 100 5 10
    240605205648_zx74 TGGGAGCTGGCATCAACAGTATGGTCCTATTG(+CAGTGT)GATCCTAGCAGACCTTTTTGCTACAGTGGAG NGG 10 8 17 8 24 0 100 5 10
    240605205647_pem3 GTATCCTCCACTGTAGCAAAAAGGTCTGCTAG(+CAGTGT)GATCCAATAGGACCATACTGTTGATGCCAGC NGG 10 8 17 8 24 0 100 5 10
    240605205646_9y6q AGAGGCTTTCTGTGCTACACTGTCTTAGCTCG(+CAGTGT)GATCCGGTGGGGGTTTGGCTGTCCTGTGGCC NGG 10 8 17 8 24 0 100 5 10
    240605205643_cpxj GGGCAGGCCACAGGACAGCCAAACCCCCACCG(+CAGTGT)GATCCGAGCTAAGACAGTGTAGCACAGAAAG NGG 10 8 17 8 24 0 100 5 10
    240605205641_51a5 CCTGGCTGGAGATGCTGCTGGGAGAGGCTGTG(+CAGTGT)GTACCCGTAGGGAGAATCCCTAGCTCCCACC NGG 10 8 17 8 24 0 100 5 10