Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605205614_bocq TGGAGGAAACACTCTGTTGGAACCCTCCTGTG(+CAGTGT)GATCCACACCTGACTTAGCACTTAAGCTAAC NGG 10 8 17 8 24 0 100 5 10
    240605205613_xycl GGAGGGTTAGCTTAAGTGCTAAGTCAGGTGTG(+CAGTGT)GATCCACAGGAGGGTTCCAACAGAGTGTTTC NGG 10 8 17 8 24 0 100 5 10
    240605205610_q1u9 TTGGGACCTGGCATCAGGACCTCTCCACCCAG(+CAGTGT)GATCCTCCATGCCACATGGTCACTGCTCTCC NGG 10 8 17 8 24 0 100 5 10
    240605205609_tc69 CCTGAGGAGAGCAGTGACCATGTGGCATGGAG(+CAGTGT)GATCCTGGGTGGAGAGGTCCTGATGCCAGGT NGG 10 8 17 8 24 0 100 5 10
    240605205608_060h GGCTGTGCTCTTAGGCTGTGCAAACATTGCAG(+CAGTGT)GTACCCCTTTCCAGTTGCGAGCCACACATCA NGG 10 8 17 8 24 0 100 5 10
    240605205605_4v6q TAGGATGATGTGTGGCTCGCAACTGGAAAGGG(+CAGTGT)GTACCTGCAATGTTTGCACAGCCTAAGAGCA NGG 10 8 17 8 24 0 100 5 10
    240605205604_wftd AGGGATGTCAGATAACTTGACCCAGGGCTATG(+CAGTGT)GATCCACTGTGAAGGATGGCTTCCCAGAGTC NGG 10 8 17 8 24 0 100 5 10
    240605205603_emvx CAGAAGACTCTGGGAAGCCATCCTTCACAGTG(+CAGTGT)GATCCATAGCCCTGGGTCAAGTTATCTGACA NGG 10 8 17 8 24 0 100 5 10
    240605205602_cw8h TTTCTGGGCTCTGCTGGAAGGGCCCTGAGATG(+CAGTGT)GTACCCGACCTGTGGAGACTGAGAGGGTACT NGG 10 8 17 8 24 0 100 5 10
    240605205600_ws7u GTAACAGTACCCTCTCAGTCTCCACAGGTCGG(+CAGTGT)GTACCATCTCAGGGCCCTTCCAGCAGAGCCC NGG 10 8 17 8 24 0 100 5 10