Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605205624_zygp CAGGGACGAGTCGGCTAATAAATAGAGGGTAG(+CAGTGT)GTACCAGCAGGGAGGGTTCTGAGAGGGTGAC NGG 10 8 17 8 24 0 100 5 10
    240605205622_nfm5 TGGCTCAGTAGACCCAAACCAGGACTGTCCTG(+CAGTGT)GATCCACTTTAGTCATGCTACCAGTGGGCTG NGG 10 8 17 8 24 0 100 5 10
    240605205621_8jev TGTAGCAGCCCACTGGTAGCATGACTAAAGTG(+CAGTGT)GATCCAGGACAGTCCTGGTTTGGGTCTACTG NGG 10 8 17 8 24 0 100 5 10
    240605205620_exat TCAAAAAAGCCCCAAGAAATTTTGAAGTGTGG(+CAGTGT)GTACCTCGTTGATTCTTACGCCAGGGCACAG NGG 10 8 17 8 24 0 100 5 10
    240605205618_tm9v TGATCCTGTGCCCTGGCGTAAGAATCAACGAG(+CAGTGT)GTACCCACACTTCAAAATTTCTTGGGGCTTT NGG 10 8 17 8 24 0 100 5 10
    240605205617_9mr4 CATTTTCCAGTATAATAAAGGTAGCTAAGATG(+CAGTGT)GATCCATATGCAGAAATAGATTACAGTCCTT NGG 10 8 17 8 24 0 100 5 10
    240605205616_dcs2 TGGCTAAGGACTGTAATCTATTTCTGCATATG(+CAGTGT)GATCCATCTTAGCTACCTTTATTATACTGGA NGG 10 8 17 8 24 0 100 5 10
    240605205614_bocq TGGAGGAAACACTCTGTTGGAACCCTCCTGTG(+CAGTGT)GATCCACACCTGACTTAGCACTTAAGCTAAC NGG 10 8 17 8 24 0 100 5 10
    240605205613_xycl GGAGGGTTAGCTTAAGTGCTAAGTCAGGTGTG(+CAGTGT)GATCCACAGGAGGGTTCCAACAGAGTGTTTC NGG 10 8 17 8 24 0 100 5 10
    240605205610_q1u9 TTGGGACCTGGCATCAGGACCTCTCCACCCAG(+CAGTGT)GATCCTCCATGCCACATGGTCACTGCTCTCC NGG 10 8 17 8 24 0 100 5 10