Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605205542_uq7v ATGGTGGCTTCAGACTCTCTCCAGTTCCAGGG(+CAGTGT)GATCCAATGCCCCAATACCCTCTCCAGCCAC NGG 10 8 17 8 24 0 100 5 10
    240605205540_ta21 GGAGAGTGGCTGGAGAGGGTATTGGGGCATTG(+CAGTGT)GATCCCCTGGAACTGGAGAGAGTCTGAAGCC NGG 10 8 17 8 24 0 100 5 10
    240605205538_of2v AGTGTAGGACTGGAGACTGAGAAGGGGTTTGG(+CAGTGT)GTACCCGCGCTCTTCCGAGGAAGTGCTGTTA NGG 10 8 17 8 24 0 100 5 10
    240605205537_9n7x TCTCTTAACAGCACTTCCTCGGAAGAGCGCGG(+CAGTGT)GTACCCAAACCCCTTCTCAGTCTCCAGTCCT NGG 10 8 17 8 24 0 100 5 10
    240605205535_xfxe GCTTGCTCCGTTGCGCACTTTGGATCTCTGGG(+CAGTGT)GATCCCTTTCTTCTTGGCTCACTGAGATGAT NGG 10 8 17 8 24 0 100 5 10
    240605205533_qsm3 CCTACATCATCTCAGTGAGCCAAGAAGAAAGG(+CAGTGT)GATCCCCAGAGATCCAAAGTGCGCAACGGAG NGG 10 8 17 8 24 0 100 5 10
    240605205532_uq83 GGCTCAACGCTGGAATCAGTTTCCCTGAAAAG(+CAGTGT)GATCCTCGGACTAAAAGTGGCATGTTAGAAA NGG 10 8 17 8 24 0 100 5 10
    240605205530_3bil GGTTTTTTCTAACATGCCACTTTTAGTCCGAG(+CAGTGT)GATCCTTTTCAGGGAAACTGATTCCAGCGTT NGG 10 8 17 8 24 0 100 5 10
    240605205529_wvm3 GAACGACAACCTGAGAGAAGCAGGTGGACTGG(+CAGTGT)GTACCTTCTGCTCTCCACTGGCCATTGGCAG NGG 10 8 17 8 24 0 100 5 10
    240605205527_be5c CACTCCTGCCAATGGCCAGTGGAGAGCAGAAG(+CAGTGT)GTACCCAGTCCACCTGCTTCTCTCAGGTTGT NGG 10 8 17 8 24 0 100 5 10