Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605205431_qii6 TCCTGCCTGGCTACATGAAGTGGTCACTTGAG(+CAGTGT)GATCCATTCCACCCACTACTAGAAGTCTCAG NGG 10 8 17 8 24 0 100 5 10
    240605205429_vgo8 TTTAGCTGAGACTTCTAGTAGTGGGTGGAATG(+CAGTGT)GATCCTCAAGTGACCACTTCATGTAGCCAGG NGG 10 8 17 8 24 0 100 5 10
    240605205428_05qz ATTTCCTAAAACAATTAAGAAATTTTGCTATG(+CAGTGT)GATCCAAGTTTCCTTACCCATAGATCTGACA NGG 10 8 17 8 24 0 100 5 10
    240605205427_bng4 CATCATGTCAGATCTATGGGTAAGGAAACTTG(+CAGTGT)GATCCATAGCAAAATTTCTTAATTGTTTTAG NGG 10 8 17 8 24 0 100 5 10
    240605205424_vwgp TGTGGGGCATTTCTTATGCCCTGCCCCCAAGG(+CAGTGT)GATCCAAGTCACCACATTGCCAAAGAATCCA NGG 10 8 17 8 24 0 100 5 10
    240605205422_hqnw AAGCCTGGATTCTTTGGCAATGTGGTGACTTG(+CAGTGT)GATCCCTTGGGGGCAGGGCATAAGAAATGCC NGG 10 8 17 8 24 0 100 5 10
    240605205421_9omc TGGCCAACAAGGTGGCTGACCCAATCGGTGAG(+CAGTGT)GATCCAAGAGATGGAGGGAGAGCACGAAAGT NGG 10 8 17 8 24 0 100 5 10
    240605205420_p5dm GTTTTACTTTCGTGCTCTCCCTCCATCTCTTG(+CAGTGT)GATCCTCACCGATTGGGTCAGCCACCTTGTT NGG 10 8 17 8 24 0 100 5 10
    240605205418_02y3 TTAAACCATTTTATGAGTACCATTCTGCAAAG(+CAGTGT)GATCCCATAGTGGTGCACAGCCTTAAGAAGC NGG 10 8 17 8 24 0 100 5 10
    240605205416_eb9o GCCTGGCTTCTTAAGGCTGTGCACCACTATGG(+CAGTGT)GATCCTTTGCAGAATGGTACTCATAAAATGG NGG 10 8 17 8 24 0 100 5 10