Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605205359_ne1a ACTTTGGCAGGACTCTTCCCAGAGGGTCACAG(+CAGTGT)GATCCAAGGCCAGGTCTGTAAATAGCAGTGT NGG 10 8 17 8 24 0 100 5 10
    240605205357_r5o7 TATACACACTGCTATTTACAGACCTGGCCTTG(+CAGTGT)GATCCTGTGACCCTCTGGGAAGAGTCCTGCC NGG 10 8 17 8 24 0 100 5 10
    240605205354_dy2v GTACTGGGGAGGAAACTAATCCAACTTCCCAG(+CAGTGT)GATCCTCTACCACCCCCATGTTCAGCTCTCT NGG 10 8 17 8 24 0 100 5 10
    240605205353_bg5w GGGCAAGAGAGCTGAACATGGGGGTGGTAGAG(+CAGTGT)GATCCTGGGAAGTTGGATTAGTTTCCTCCCC NGG 10 8 17 8 24 0 100 5 10
    240605205352_tdw3 TTTGTGTTAAAATCTGTGAAGTATTAAGACTG(+CAGTGT)GTACCAGATTTCACAAGAAAAATGTAATTTC NGG 10 8 17 8 24 0 100 5 10
    240605205350_snaw AGATGGAAATTACATTTTTCTTGTGAAATCTG(+CAGTGT)GTACCAGTCTTAATACTTCACAGATTTTAAC NGG 10 8 17 8 24 0 100 5 10
    240605205349_in2e TAATGTACAGCGAGGCCCAGAGGTGTACAGTG(+CAGTGT)GATCCACAGCGGGGGGAAAAAAGGCAGAGTT NGG 10 8 17 8 24 0 100 5 10
    240605205347_79b1 CCTAGAACTCTGCCTTTTTTCCCCCCGCTGTG(+CAGTGT)GATCCACTGTACACCTCTGGGCCTCGCTGTA NGG 10 8 17 8 24 0 100 5 10
    240605205345_hf2f GGAGCAGGGACCTGATCTCCTATCTGTCCTGG(+CAGTGT)GATCCATTTTCAGAGGGGCAGTTTTATTTGG NGG 10 8 17 8 24 0 100 5 10
    240605205344_vqeh CCAGGCCAAATAAAACTGCCCCTCTGAAAATG(+CAGTGT)GATCCCAGGACAGATAGGAGATCAGGTCCCT NGG 10 8 17 8 24 0 100 5 10