Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605211124_gx3x ATGGGGTTACCCTCATCAGATCTGTAGCTTGG(+CAGTGT)GTACCCATCCTAACACCTGTTCCTTCCCTGG NGG 10 8 17 8 24 0 100 5 10
    240605211122_kk5w CACGGCAAGAGCAAGGCTGCAAACTGTCTCTG(+CAGTGT)GTACCACGGAATGTGCTTTCCAACACACATG NGG 10 8 17 8 24 0 100 5 10
    240605211120_jmdg CAGCCCATGTGTGTTGGAAAGCACATTCCGTG(+CAGTGT)GTACCAGAGACAGTTTGCAGCCTTGCTCTTG NGG 10 8 17 8 24 0 100 5 10
    240605211119_33p4 GTTCCTCCACAGATGTGCTAAAGTCCAAAGAG(+CAGTGT)GATCCATTTGGAAGGAGTGGCTTGGCCGGGG NGG 10 8 17 8 24 0 100 5 10
    240605211117_e47m CACGGCCCCGGCCAAGCCACTCCTTCCAAATG(+CAGTGT)GATCCTCTTTGGACTTTAGCACATCTGTGGA NGG 10 8 17 8 24 0 100 5 10
    240605211116_dbau GAGTGGGTAGTTGTGGACTTGTTAACAGTAAG(+CAGTGT)GTACCTTTGAAGTATGCAGATGGAGGTGACC NGG 10 8 17 8 24 0 100 5 10
    240605211114_1mfh CACTGGGTCACCTCCATCTGCATACTTCAAAG(+CAGTGT)GTACCTTACTGTTAACAAGTCCACAACTACC NGG 10 8 17 8 24 0 100 5 10
    240605211113_2fcr GACCGAGCTATGCATGGAGGAGGAGGCTGTTG(+CAGTGT)GTACCATACTGAATTTCCACTGGACCTCTAA NGG 10 8 17 8 24 0 100 5 10
    240605211112_9cf6 TGACTTTAGAGGTCCAGTGGAAATTCAGTATG(+CAGTGT)GTACCAACAGCCTCCTCCTCCATGCATAGCT NGG 10 8 17 8 24 0 100 5 10
    240605211110_1r7k GTGGGCATTCATAGTCTAAATCCTCTAGGGAG(+CAGTGT)GATCCTCTTGCCATCTGCCCTCCTCAGTGGC NGG 10 8 17 8 24 0 100 5 10