Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605205311_xkzj GCTGTCTTCAGACACATCAGAAGAGGGTATCG(+CAGTGT)GATCCCATTACAGATGTGGTTGCTGGGAGTT NGG 10 8 17 8 24 0 100 5 10
    240605205309_f0wu AATTCAACTCCCAGCAACCACATCTGTAATGG(+CAGTGT)GATCCGATACCCTCTTCTGATGTGTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240605205307_r2f9 TAGGTAGCCTTGGCTGGCCTGGAACTCACAGG(+CAGTGT)GATCCACCTGTCTCTGCCACCCAAGTGCTGG NGG 10 8 17 8 24 0 100 5 10
    240605205306_4g5s CAATTCCAGCACTTGGGTGGCAGAGACAGGTG(+CAGTGT)GATCCCTGTGAGTTCCAGGCCAGCCAAGGCT NGG 10 8 17 8 24 0 100 5 10
    240605205305_tey2 CAGGCAGACAGAGTAATGGAGAAGGAGCTGAG(+CAGTGT)GATCCTACGTCTTGATCTGAAAGTGGTCAGG NGG 10 8 17 8 24 0 100 5 10
    240605205303_31kj TTTCTCCTGACCACTTTCAGATCAAGACGTAG(+CAGTGT)GATCCTCAGCTCCTTCTCCATTACTCTGTCT NGG 10 8 17 8 24 0 100 5 10
    240605205302_4e7f ATCAATGCTCTCCCAATAAACGTGTGCAGAAG(+CAGTGT)GATCCTGTTGCAGCGTCGTTCTTCCTGGCCA NGG 10 8 17 8 24 0 100 5 10
    240605205301_o4mv TCAACTGGCCAGGAAGAACGACGCTGCAACAG(+CAGTGT)GATCCTTCTGCACACGTTTATTGGGAGAGCA NGG 10 8 17 8 24 0 100 5 10
    240605205300_oj4l TGAAATGGTTCAACTCCTACTGATATGGCTTG(+CAGTGT)GATCCCTCCCCTCCCCCCATGATGGTGACAT NGG 10 8 17 8 24 0 100 5 10
    240605205258_izhz GAACCATGTCACCATCATGGGGGGAGGGGAGG(+CAGTGT)GATCCAAGCCATATCAGTAGGAGTTGAACCA NGG 10 8 17 8 24 0 100 5 10