Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605205239_vtv0 AATGTAAGCTAATTATGGATATTTTCATTTAG(+CAGTGT)GTACCTTTGTTCTCTTGTTTTTTAAAGGTAT NGG 10 8 17 8 24 0 100 5 10
    240605205238_j07r TACGCATACCTTTAAAAAACAAGAGAACAAAG(+CAGTGT)GTACCTAAATGAAAATATCCATAATTAGCTT NGG 10 8 17 8 24 0 100 5 10
    240605205236_xch1 ACAACTTAGAAATGTCAGAGAACATCCTTTCG(+CAGTGT)GATCCTGTGGAGTAAGAGAGAGAGCACTAAG NGG 10 8 17 8 24 0 100 5 10
    240605205234_76eu TATCGCTTAGTGCTCTCTCTCTTACTCCACAG(+CAGTGT)GATCCGAAAGGATGTTCTCTGACATTTCTAA NGG 10 8 17 8 24 0 100 5 10
    240605205233_5p2v CGATGTCAATAAAGTGTGGTCCCTGCCATCGG(+CAGTGT)GATCCACGCAAACTTTCAGAAAAGTCTTTTT NGG 10 8 17 8 24 0 100 5 10
    240605205231_s2mp AAACTAAAAAGACTTTTCTGAAAGTTTGCGTG(+CAGTGT)GATCCCGATGGCAGGGACCACACTTTATTGA NGG 10 8 17 8 24 0 100 5 10
    240605205229_goee AGACACGTAAGTAGACAGATAAACAGACAGAG(+CAGTGT)GTACCAGTCTTTCACTATACCCAAGGTAGTC NGG 10 8 17 8 24 0 100 5 10
    240605205227_w7xi TCTCAGACTACCTTGGGTATAGTGAAAGACTG(+CAGTGT)GTACCTCTGTCTGTTTATCTGTCTACTTACG NGG 10 8 17 8 24 0 100 5 10
    240605205226_td52 AATCTTATGTGTAGACAAGGACACACACACAG(+CAGTGT)GTACCTATGCATTCATATACAGTAGACCAAT NGG 10 8 17 8 24 0 100 5 10
    240605205223_hjhz TTCCCATTGGTCTACTGTATATGAATGCATAG(+CAGTGT)GTACCTGTGTGTGTGTCCTTGTCTACACATA NGG 10 8 17 8 24 0 100 5 10