Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605205125_kwba TGACAGTAACTGATCTGCATTAGTTAGGAGTG(+CAGTGT)GTACCGCTTTCATCAGTTCAATGATGCTACT NGG 10 8 17 8 24 0 100 5 10
    240605205124_tody TGTAAAGTAGCATCATTGAACTGATGAAAGCG(+CAGTGT)GTACCACTCCTAACTAATGCAGATCAGTTAC NGG 10 8 17 8 24 0 100 5 10
    240605205122_4crg GAAGAACTCTTATGAATACATTAAAGTAAAAG(+CAGTGT)GATCCCTAAAACGATTAAGCATACAGGTTGG NGG 10 8 17 8 24 0 100 5 10
    240605205121_k09u ATTGTCCAACCTGTATGCTTAATCGTTTTAGG(+CAGTGT)GATCCTTTTACTTTAATGTATTCATAAGAGT NGG 10 8 17 8 24 0 100 5 10
    240605205120_gq7q TTCTGTGCCTCAAGGAAAAGGAGCCCTCTTAG(+CAGTGT)GTACCGCCACAACATGGATGATCCAGACTGT NGG 10 8 17 8 24 0 100 5 10
    240605205118_oryo AGGCAACAGTCTGGATCATCCATGTTGTGGCG(+CAGTGT)GTACCTAAGAGGGCTCCTTTTCCTTGAGGCA NGG 10 8 17 8 24 0 100 5 10
    240605205117_fhg3 TTGGATCCAGTGACATAGCTAATAGGGTGAAG(+CAGTGT)GTACCTGCCACCAACTCTGATAATTTGAGTT NGG 10 8 17 8 24 0 100 5 10
    240605205115_sohy GATTGAACTCAAATTATCAGAGTTGGTGGCAG(+CAGTGT)GTACCTTCACCCTATTAGCTATGTCACTGGA NGG 10 8 17 8 24 0 100 5 10
    240605205113_yhnm TTTTATTCCTATCAAAAATGTCTTTATGCCTG(+CAGTGT)GATCCATCATCAAATAGTGTTCAATTCACTG NGG 10 8 17 8 24 0 100 5 10
    240605205112_xac2 CAAACCAGTGAATTGAACACTATTTGATGATG(+CAGTGT)GATCCAGGCATAAAGACATTTTTGATAGGAA NGG 10 8 17 8 24 0 100 5 10