Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605205054_9va0 TTGAAATTGTACAGTGACGAAGATGTGACCAG(+CAGTGT)GATCCAGAGGTTCCTGGGGAAGATCTGCCTT NGG 10 8 17 8 24 0 100 5 10
    240605205052_80vq TTCACAAGGCAGATCTTCCCCAGGAACCTCTG(+CAGTGT)GATCCTGGTCACATCTTCGTCACTGTACAAT NGG 10 8 17 8 24 0 100 5 10
    240605205050_ifps GAAATAAAGTTGGTGACAGATGCCACGAAGGG(+CAGTGT)GTACCCTCCAAACCCTCACAGCAAGAAAGAG NGG 10 8 17 8 24 0 100 5 10
    240605205048_8wdn TATTTCTCTTTCTTGCTGTGAGGGTTTGGAGG(+CAGTGT)GTACCCCTTCGTGGCATCTGTCACCAACTTT NGG 10 8 17 8 24 0 100 5 10
    240605205047_8e63 AGCAACGGCAGGGATTTCGAAAAGTAAGACAG(+CAGTGT)GATCCACAGACAATAGAAGCATATGGGTGAC NGG 10 8 17 8 24 0 100 5 10
    240605205046_h7ov GTGACGTCACCCATATGCTTCTATTGTCTGTG(+CAGTGT)GATCCTGTCTTACTTTTCGAAATCCCTGCCG NGG 10 8 17 8 24 0 100 5 10
    240605205043_0aaf TAGGACCAATACACCAAACTAAAAGGGGAGGG(+CAGTGT)GTACCCTGTGGACTTTATTTAGCAACCTAAG NGG 10 8 17 8 24 0 100 5 10
    240605205042_lruh GCTCTCTTAGGTTGCTAAATAAAGTCCACAGG(+CAGTGT)GTACCCCTCCCCTTTTAGTTTGGTGTATTGG NGG 10 8 17 8 24 0 100 5 10
    240605205040_9ycp AATTATATTTTAACTGTTTTATAAAAACTAGG(+CAGTGT)GTACCTGTATCACAGCAAATCACTGAATTTA NGG 10 8 17 8 24 0 100 5 10
    240605205038_iy55 ATTCTTAAATTCAGTGATTTGCTGTGATACAG(+CAGTGT)GTACCCTAGTTTTTATAAAACAGTTAAAATA NGG 10 8 17 8 24 0 100 5 10