Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605205043_0aaf TAGGACCAATACACCAAACTAAAAGGGGAGGG(+CAGTGT)GTACCCTGTGGACTTTATTTAGCAACCTAAG NGG 10 8 17 8 24 0 100 5 10
    240605205042_lruh GCTCTCTTAGGTTGCTAAATAAAGTCCACAGG(+CAGTGT)GTACCCCTCCCCTTTTAGTTTGGTGTATTGG NGG 10 8 17 8 24 0 100 5 10
    240605205040_9ycp AATTATATTTTAACTGTTTTATAAAAACTAGG(+CAGTGT)GTACCTGTATCACAGCAAATCACTGAATTTA NGG 10 8 17 8 24 0 100 5 10
    240605205038_iy55 ATTCTTAAATTCAGTGATTTGCTGTGATACAG(+CAGTGT)GTACCCTAGTTTTTATAAAACAGTTAAAATA NGG 10 8 17 8 24 0 100 5 10
    240605205037_7vlj CCTTAAAGCAGCAGTCATTTATAGCAACTCTG(+CAGTGT)GTACCAACGGCAAAGGGGGGAAAACTGGTTT NGG 10 8 17 8 24 0 100 5 10
    240605205036_xcmg TTTTTAAACCAGTTTTCCCCCCTTTGCCGTTG(+CAGTGT)GTACCAGAGTTGCTATAAATGACTGCTGCTT NGG 10 8 17 8 24 0 100 5 10
    240605205034_kw8e CCCTGAACTCAGAAACTGAAATTTAAATTCTG(+CAGTGT)GATCCTTTGGGTGGGAGAGATGGCTCAGTGA NGG 10 8 17 8 24 0 100 5 10
    240605205032_kb3a CTTAATCACTGAGCCATCTCTCCCACCCAAAG(+CAGTGT)GATCCAGAATTTAAATTTCAGTTTCTGAGTT NGG 10 8 17 8 24 0 100 5 10
    240605205030_oukf ATCTTACACAGGGGCACTATTACTTTCCACGG(+CAGTGT)GTACCAAGAGCGCTCCGGCTGAATCACACCC NGG 10 8 17 8 24 0 100 5 10
    240605205029_aj3u TGGAAGGGTGTGATTCAGCCGGAGCGCTCTTG(+CAGTGT)GTACCCGTGGAAAGTAATAGTGCCCCTGTGT NGG 10 8 17 8 24 0 100 5 10