Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605204939_g4wu GTGTCAAGTTTCTTTCCACATCCTGTCCAAAG(+CAGTGT)GTACCCAGGAAAAATTCCACTTTCTTATAAT NGG 10 8 17 8 24 0 100 5 10
    240605204936_m5xf AGTCTATTATAAGAAAGTGGAATTTTTCCTGG(+CAGTGT)GTACCTTTGGACAGGATGTGGAAAGAAACTT NGG 10 8 17 8 24 0 100 5 10
    240605204935_0mg6 TTTTCTGCCAAATAACGTTGACCAGTTATATG(+CAGTGT)GTACCAAGAAATGCCCCTGCCCCTCCCTATA NGG 10 8 17 8 24 0 100 5 10
    240605204933_im4o TGATCTATAGGGAGGGGCAGGGGCATTTCTTG(+CAGTGT)GTACCATATAACTGGTCAACGTTATTTGGCA NGG 10 8 17 8 24 0 100 5 10
    240605204931_322p TCATATGTGCAGCTGCCCTGCCATGTCCAGAG(+CAGTGT)GATCCTGCTTCCTCGTATTCGTTCACCACCT NGG 10 8 17 8 24 0 100 5 10
    240605204929_9wev GACAGAGGTGGTGAACGAATACGAGGAAGCAG(+CAGTGT)GATCCTCTGGACATGGCAGGGCAGCTGCACA NGG 10 8 17 8 24 0 100 5 10
    240605204928_kbsz TGGAATTATGAAGGTACTGGTTTATGGGTGAG(+CAGTGT)GATCCTTGGCATTATATTTTGGCTGGCCTCT NGG 10 8 17 8 24 0 100 5 10
    240605204926_pkyx AAGAAAGAGGCCAGCCAAAATATAATGCCAAG(+CAGTGT)GATCCTCACCCATAAACCAGTACCTTCATAA NGG 10 8 17 8 24 0 100 5 10
    240605204925_aes7 CTGGGCAGGAGCAGCCAGCCCTCAGGAGGCTG(+CAGTGT)GTACCAAAGGCATGAATGTTCTCAGGGGATA NGG 10 8 17 8 24 0 100 5 10
    240605204923_bep9 CATCTTATCCCCTGAGAACATTCATGCCTTTG(+CAGTGT)GTACCAGCCTCCTGAGGGCTGGCTGCTCCTG NGG 10 8 17 8 24 0 100 5 10