Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605204921_f41m TGAATTCCATGTGTGTTTTATGACCTAAATGG(+CAGTGT)GATCCTGACACCTGCTGTGTGGCCACCACGA NGG 10 8 17 8 24 0 100 5 10
    240605204920_tccv AAGAATCGTGGTGGCCACACAGCAGGTGTCAG(+CAGTGT)GATCCCATTTAGGTCATAAAACACACATGGA NGG 10 8 17 8 24 0 100 5 10
    240605204914_kwdc AGACATATCCCTGGGCTCATGTGAAATGCCAG(+CAGTGT)GTACCCAGGACAGGTTCTCCCACCATTTCTA NGG 10 8 17 8 24 0 100 5 10
    240605204911_q8sf GGGCATAGAAATGGTGGGAGAACCTGTCCTGG(+CAGTGT)GTACCTGGCATTTCACATGAGCCCAGGGATA NGG 10 8 17 8 24 0 100 5 10
    240605204910_eoai CTCCCCAGCACTGCAGGAGGCCGCATTTCATG(+CAGTGT)GATCCACGCATAAAGCTCAAGTGATAGCAGT NGG 10 8 17 8 24 0 100 5 10
    240605204909_5163 GAGCAACTGCTATCACTTGAGCTTTATGCGTG(+CAGTGT)GATCCATGAAATGCGGCCTCCTGCAGTGCTG NGG 10 8 17 8 24 0 100 5 10
    240605204907_wcrb GGCGTTGTGTGCATAGCAGTCTAGCCGGTTGG(+CAGTGT)GTACCCTGCTCCTGTACCTAGTTCACAGACT NGG 10 8 17 8 24 0 100 5 10
    240605204905_dymi ACTCGAGTCTGTGAACTAGGTACAGGAGCAGG(+CAGTGT)GTACCCAACCGGCTAGACTGCTATGCACACA NGG 10 8 17 8 24 0 100 5 10
    240605204903_u32t CGGGCGCTGACCGCGGGGACTTGACCAGTAGG(+CAGTGT)GATCCAGGTCGGTGCCCCACGCAAGACAGGC NGG 10 8 17 8 24 0 100 5 10
    240605204901_b3oc GCAGTGCCTGTCTTGCGTGGGGCACCGACCTG(+CAGTGT)GATCCCTACTGGTCAAGTCCCCGCGGTCAGC NGG 10 8 17 8 24 0 100 5 10