Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605204810_znsp CCGATACACCAGGTCATTCCTCTGCCCACTTG(+CAGTGT)GTACCTGGTGTACTAAAGGACTGCATGTCTT NGG 10 8 17 8 24 0 100 5 10
    240605204808_uqb3 CTGCAAAGACATGCAGTCCTTTAGTACACCAG(+CAGTGT)GTACCAAGTGGGCAGAGGAATGACCTGGTGT NGG 10 8 17 8 24 0 100 5 10
    240605204806_ti93 CTTGACAGGGCCTGGCGCATGGGGAGTGCAGG(+CAGTGT)GATCCAGGACCCCCATCCCCAACCCGATACA NGG 10 8 17 8 24 0 100 5 10
    240605204804_odrz CCTGGTGTATCGGGTTGGGGATGGGGGTCCTG(+CAGTGT)GATCCCTGCACTCCCCATGCGCCAGGCCCTG NGG 10 8 17 8 24 0 100 5 10
    240605204802_xhm0 ACTCCCCATGCTCCAGGCCCTGTCAAGTGCAG(+CAGTGT)GTACCTGCACTAACTCCAGCATTTCACTGCA NGG 10 8 17 8 24 0 100 5 10
    240605204801_anc6 TCAGCTGCAGTGAAATGCTGGAGTTAGTGCAG(+CAGTGT)GTACCTGCACTTGACAGGGCCTGGAGCATGG NGG 10 8 17 8 24 0 100 5 10
    240605204759_i5e3 CCTGGTGTATGGGGTCGGGAATGGGGGTCCTG(+CAGTGT)GATCCCTGCACTCCCCATGCTCCAGGCCCTG NGG 10 8 17 8 24 0 100 5 10
    240605204757_dbtt CTTGACAGGGCCTGGAGCATGGGGAGTGCAGG(+CAGTGT)GATCCAGGACCCCCATTCCCGACCCCATACA NGG 10 8 17 8 24 0 100 5 10
    240605204756_lwms ACTCCCCATGCTCCAGGCCCTGTCAAGTGCAG(+CAGTGT)GTACCTGCACTATCTCCAGCATTTCACTGCA NGG 10 8 17 8 24 0 100 5 10
    240605204754_hre1 TCAGCTGCAGTGAAATGCTGGAGATAGTGCAG(+CAGTGT)GTACCTGCACTTGACAGGGCCTGGAGCATGG NGG 10 8 17 8 24 0 100 5 10