Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605204845_c0c7 TCAGCTGCAGTGAAATGCTGGAGATAGTGCAG(+CAGTGT)GTACCTGCACTTGACAGGGCCTGGCACATGG NGG 10 8 17 8 24 0 100 5 10
    240605204844_7lhl ACTCCCCATGTGCCAGGCCCTGTCAAGTGCAG(+CAGTGT)GTACCTGCACTATCTCCAGCATTTCACTGCA NGG 10 8 17 8 24 0 100 5 10
    240605204842_cwcx TCAGCTGCAGTGAAATGCTGGAGATAGTGCAG(+CAGTGT)GTACCTGCACTTGACAGGGCCTGGTGCATGG NGG 10 8 17 8 24 0 100 5 10
    240605204841_xl5u ACTCCCCATGCACCAGGCCCTGTCAAGTGCAG(+CAGTGT)GTACCTGCACTATCTCCAGCATTTCACTGCA NGG 10 8 17 8 24 0 100 5 10
    240605204838_x3tq ACAGCCTGTCTCTGCTCCCCATGAATCCTAGG(+CAGTGT)GATCCCCATGCAGGTTCACAAGCTTGTGTAG NGG 10 8 17 8 24 0 100 5 10
    240605204837_7p1j GCTTGCTACACAAGCTTGTGAACCTGCATGGG(+CAGTGT)GATCCCTAGGATTCATGGGGAGCAGAGACAG NGG 10 8 17 8 24 0 100 5 10
    240605204835_oodd TCAGCTGCAGTGAAATGCTGGAGATAGTGCAG(+CAGTGT)GTACCTGCACTTGACAGGGCCTTAAGCATGG NGG 10 8 17 8 24 0 100 5 10
    240605204834_oejp ACTCCCCATGCTTAAGGCCCTGTCAAGTGCAG(+CAGTGT)GTACCTGCACTATCTCCAGCATTTCACTGCA NGG 10 8 17 8 24 0 100 5 10
    240605204832_ajip CTTGACAGGGCCTTAAGCATGGGGAGTGCAGG(+CAGTGT)GATCCAGGACCCCCATCCCCGGCCCCATACA NGG 10 8 17 8 24 0 100 5 10
    240605204830_zcwn CCTGGTGTATGGGGCCGGGGATGGGGGTCCTG(+CAGTGT)GATCCCTGCACTCCCCATGCTTAAGGCCCTG NGG 10 8 17 8 24 0 100 5 10