Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605204733_6z3i TATGCAGAATCCTTGGGAAGCTAGAATATGGG(+CAGTGT)GTACCATCCTCATAGCCCCAGAGAGTGGGAG NGG 10 8 17 8 24 0 100 5 10
    240605204731_93yz GGCTTCTCCCACTCTCTGGGGCTATGAGGATG(+CAGTGT)GTACCCCATATTCTAGCTTCCCAAGGATTCT NGG 10 8 17 8 24 0 100 5 10
    240605204730_zama AGTGAGCCATCTGTGAGATCTCCATAGTCTAG(+CAGTGT)GATCCACTGGTCCCAGGGGGACTGAGTGACT NGG 10 8 17 8 24 0 100 5 10
    240605204728_s6kw CTATCAGTCACTCAGTCCCCCTGGGACCAGTG(+CAGTGT)GATCCTAGACTATGGAGATCTCACAGATGGC NGG 10 8 17 8 24 0 100 5 10
    240605204726_pi32 GAGGGTCCAGCCACCACTGTCTTCTTTCTCTG(+CAGTGT)GATCCAGAACAAGGAAATCTAGATGAGCCGG NGG 10 8 17 8 24 0 100 5 10
    240605204725_3dc5 GCCACCCGGCTCATCTAGATTTCCTTGTTCTG(+CAGTGT)GATCCAGAGAAAGAAGACAGTGGTGGCTGGA NGG 10 8 17 8 24 0 100 5 10
    240605204724_bivs GTGTGCAGTTCAATCCCGAGGACTGGAATTTG(+CAGTGT)GATCCCACCACACACATAACAAGCCACATAT NGG 10 8 17 8 24 0 100 5 10
    240605204722_u6tz ACAGAATATGTGGCTTGTTATGTGTGTGGTGG(+CAGTGT)GATCCAAATTCCAGTCCTCGGGATTGAACTG NGG 10 8 17 8 24 0 100 5 10
    240605204720_w6t9 GCCCCAAGGTCACGTGTTAGGATACTATATGG(+CAGTGT)GTACCATACATTTCAAAGTTGCTCATGGAAA NGG 10 8 17 8 24 0 100 5 10
    240605204719_yrv0 GCTCATTTCCATGAGCAACTTTGAAATGTATG(+CAGTGT)GTACCCATATAGTATCCTAACACGTGACCTT NGG 10 8 17 8 24 0 100 5 10