Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605205021_hvlm GTGCAGCATGTCTCATGCATCGTATTGAACTG(+CAGTGT)GATCCCGCACAGGGAATAATGTAATCTATTG NGG 10 8 17 8 24 0 100 5 10
    240605205019_zqj8 ATCATCAATAGATTACATTATTCCCTGTGCGG(+CAGTGT)GATCCAGTTCAATACGATGCATGAGACATGC NGG 10 8 17 8 24 0 100 5 10
    240605205018_55b3 GCATGAGAGTGTGCAGTGGTGCCTGAGTCCTG(+CAGTGT)GTACCTTGAAAAGCTTTGTCTAAGAATGCAA NGG 10 8 17 8 24 0 100 5 10
    240605205016_dswt TGTTGTTGCATTCTTAGACAAAGCTTTTCAAG(+CAGTGT)GTACCAGGACTCAGGCACCACTGCACACTCT NGG 10 8 17 8 24 0 100 5 10
    240605205015_36du GCAGCTGGTTCTCTCAGCATGTGGGTCTCCAG(+CAGTGT)GTACCAAAAGGCATGAGGCTTGGCAGCAAGC NGG 10 8 17 8 24 0 100 5 10
    240605205013_earm AAACCGCTTGCTGCCAAGCCTCATGCCTTTTG(+CAGTGT)GTACCTGGAGACCCACATGCTGAGAGAACCA NGG 10 8 17 8 24 0 100 5 10
    240605205011_tih9 TTACTTGATACACCGTGGAATGAAAATGTAAG(+CAGTGT)GTACCTTTCAGTATGTGCTCTGAGCTTTAAT NGG 10 8 17 8 24 0 100 5 10
    240605205010_d61d AAAAAATTAAAGCTCAGAGCACATACTGAAAG(+CAGTGT)GTACCTTACATTTTCATTCCACGGTGTATCA NGG 10 8 17 8 24 0 100 5 10
    240605205008_bi3d CAGCAGTGAAACATTTAAATGCATATCAATGG(+CAGTGT)GTACCATGTCTAAAAATTACTATAGTACCTA NGG 10 8 17 8 24 0 100 5 10
    240605205007_vd2z CTAAATAGGTACTATAGTAATTTTTAGACATG(+CAGTGT)GTACCCATTGATATGCATTTAAATGTTTCAC NGG 10 8 17 8 24 0 100 5 10