Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605205141_wjhz AGGGAACCCAGGGTCACCTGCAGGTGACAGAG(+CAGTGT)GTACCCGCCTCCACTTAATGTCCATCAAGCT NGG 10 8 17 8 24 0 100 5 10
    240605205139_fmue ACCCCAGCTTGATGGACATTAAGTGGAGGCGG(+CAGTGT)GTACCTCTGTCACCTGCAGGTGACCCTGGGT NGG 10 8 17 8 24 0 100 5 10
    240605205137_6m0e GGTGGGCTAAGTTGCCAAAAACGACAATCAGG(+CAGTGT)GATCCAATAAGCAATCTTGGTCACTTAAGTA NGG 10 8 17 8 24 0 100 5 10
    240605205136_6dmf AAATTTACTTAAGTGACCAAGATTGCTTATTG(+CAGTGT)GATCCCTGATTGTCGTTTTTGGCAACTTAGC NGG 10 8 17 8 24 0 100 5 10
    240605205135_7b79 TTCTTAGTATTCAGAATTGATGTGTTTTATTG(+CAGTGT)GTACCAGCTGTCTTAATGCATTTATTAAGAA NGG 10 8 17 8 24 0 100 5 10
    240605205133_zm6q AACTGTTCTTAATAAATGCATTAAGACAGCTG(+CAGTGT)GTACCAATAAAACACATCAATTCTGAATACT NGG 10 8 17 8 24 0 100 5 10
    240605205132_sb77 GTCTCACACCTCAGTCCCTTGGTCACACTAAG(+CAGTGT)GTACCCAAGATGTTGGCCAGGATTACTAGAG NGG 10 8 17 8 24 0 100 5 10
    240605205130_1r1l GGAATCTCTAGTAATCCTGGCCAACATCTTGG(+CAGTGT)GTACCTTAGTGTGACCAAGGGACTGAGGTGT NGG 10 8 17 8 24 0 100 5 10
    240605205129_g3b9 AGTTAGCTGGGGCAGAGGTATGGTTGGAGTAG(+CAGTGT)GTACCTAGGACGGCAACCCCTTCGTAATAGG NGG 10 8 17 8 24 0 100 5 10
    240605205126_t1x3 ACCCCCCTATTACGAAGGGGTTGCCGTCCTAG(+CAGTGT)GTACCTACTCCAACCATACCTCTGCCCCAGC NGG 10 8 17 8 24 0 100 5 10