Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605205157_26r2 TCCTGTATCTTGCCTTTGTACAAGAGCATATG(+CAGTGT)GTACCTTTTTGAATAAAATAACATTTAACAT NGG 10 8 17 8 24 0 100 5 10
    240605205156_rmz1 TAAATATGTTAAATGTTATTTTATTCAAAAAG(+CAGTGT)GTACCATATGCTCTTGTACAAAGGCAAGATA NGG 10 8 17 8 24 0 100 5 10
    240605205154_kaea AGGAAGCTCTGGCAATTCTGGTTCTTAACCAG(+CAGTGT)GATCCAGGAGGCATCACTGGGAAAGTGTGAT NGG 10 8 17 8 24 0 100 5 10
    240605205152_3dqk TTGAAATCACACTTTCCCAGTGATGCCTCCTG(+CAGTGT)GATCCTGGTTAAGAACCAGAATTGCCAGAGC NGG 10 8 17 8 24 0 100 5 10
    240605205150_jhjs GATCGTTGAATCTCTCTCATCCCAGTGCAATG(+CAGTGT)GATCCACAAATCCGTGGGATGCAAAAGCAGA NGG 10 8 17 8 24 0 100 5 10
    240605205149_1nlc AGTTCTCTGCTTTTGCATCCCACGGATTTGTG(+CAGTGT)GATCCATTGCACTGGGATGAGAGAGATTCAA NGG 10 8 17 8 24 0 100 5 10
    240605205148_av8a ACAAATAGCTCCCCAGGATCTGATTAAGATAG(+CAGTGT)GATCCGGGCCATACCATTTATTATCAAGGAC NGG 10 8 17 8 24 0 100 5 10
    240605205145_wbkk AAAATGTCCTTGATAATAAATGGTATGGCCCG(+CAGTGT)GATCCTATCTTAATCAGATCCTGGGGAGCTA NGG 10 8 17 8 24 0 100 5 10
    240605205143_lwhi ATTTGAAATAAAGGTTTTAATTCTGAAGTAGG(+CAGTGT)GATCCTTAAATAAGGTCTAATCCAATTTATA NGG 10 8 17 8 24 0 100 5 10
    240605205142_7na9 GGAGATATAAATTGGATTAGACCTTATTTAAG(+CAGTGT)GATCCCTACTTCAGAATTAAAACCTTTATTT NGG 10 8 17 8 24 0 100 5 10