Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605205220_vazb CTGAAAACATAACTTAAATAGACTGACTGGAG(+CAGTGT)GATCCGCCACACACGTGTTCATGGATGGGAA NGG 10 8 17 8 24 0 100 5 10
    240605205218_gw4a CACTGTTCCCATCCATGAACACGTGTGTGGCG(+CAGTGT)GATCCTCCAGTCAGTCTATTTAAGTTATGTT NGG 10 8 17 8 24 0 100 5 10
    240605205216_5r9h CTGGATTGGAACTCATTAAGACCAACAGCCAG(+CAGTGT)GATCCCGATTTTGAGCCTAAACTTTTGTTCC NGG 10 8 17 8 24 0 100 5 10
    240605205215_lt5q AGGAAGGAACAAAAGTTTAGGCTCAAAATCGG(+CAGTGT)GATCCTGGCTGTTGGTCTTAATGAGTTCCAA NGG 10 8 17 8 24 0 100 5 10
    240605205214_8e7q TATAATTTATGCAGAGAAATTTCACTTACATG(+CAGTGT)GTACCTTTTCCAGTCTCAACATCTCATCATA NGG 10 8 17 8 24 0 100 5 10
    240605205212_0eb8 CATTTTATGATGAGATGTTGAGACTGGAAAAG(+CAGTGT)GTACCATGTAAGTGAAATTTCTCTGCATAAA NGG 10 8 17 8 24 0 100 5 10
    240605205211_if59 GGAAGGTTGGTCCCACAAGATGCACTGAAATG(+CAGTGT)GATCCCCACCCAATAAAGATGGTATTGTTAC NGG 10 8 17 8 24 0 100 5 10
    240605205209_j9nc CATATGTAACAATACCATCTTTATTGGGTGGG(+CAGTGT)GATCCATTTCAGTGCATCTTGTGGGACCAAC NGG 10 8 17 8 24 0 100 5 10
    240605205200_mpih TACATAAACCAATGCTTGAAGATGCTGCAAAG(+CAGTGT)GATCCTCTAGTGATTCCTGATGAGGAATCAG NGG 10 8 17 8 24 0 100 5 10
    240605205159_gp1s CTATTCTGATTCCTCATCAGGAATCACTAGAG(+CAGTGT)GATCCTTTGCAGCATCTTCAAGCATTGGTTT NGG 10 8 17 8 24 0 100 5 10