Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605205342_doub GTTAAGGAACTGGCCTTGGATTTTCTTTCTCG(+CAGTGT)GTACCCATCTCTTTTTACTTACACTCTACAG NGG 10 8 17 8 24 0 100 5 10
    240605205340_kuyg AATATCTGTAGAGTGTAAGTAAAAAGAGATGG(+CAGTGT)GTACCGAGAAAGAAAATCCAAGGCCAGTTCC NGG 10 8 17 8 24 0 100 5 10
    240605205339_8xmh CGGGAGGACCTAGTTCTCCTCTGATCTCTGAG(+CAGTGT)GTACCTGCACTTGTGCGTGCCAGACCCATGC NGG 10 8 17 8 24 0 100 5 10
    240605205337_yyk0 TCTGTGCATGGGTCTGGCACGCACAAGTGCAG(+CAGTGT)GTACCTCAGAGATCAGAGGAGAACTAGGTCC NGG 10 8 17 8 24 0 100 5 10
    240605205336_xtmy GGGAATCTGATGCTCTCCTCAGACCTCTGAAG(+CAGTGT)GTACCAGACATGTACTTAGTAAACAGACATG NGG 10 8 17 8 24 0 100 5 10
    240605205334_1710 GCATGCATGTCTGTTTACTAAGTACATGTCTG(+CAGTGT)GTACCTTCAGAGGTCTGAGGAGAGCATCAGA NGG 10 8 17 8 24 0 100 5 10
    240605205333_j5y9 GAGAGGTAGAATTGGCCACCTGGGGGGACGTG(+CAGTGT)GTACCAGAAGTAGTCGGGATACCTTTAGAGT NGG 10 8 17 8 24 0 100 5 10
    240605205331_ud2t CGGCTACTCTAAAGGTATCCCGACTACTTCTG(+CAGTGT)GTACCACGTCCCCCCAGGTGGCCAATTCTAC NGG 10 8 17 8 24 0 100 5 10
    240605205330_r05j CACACACCAAAGCTGTTTGCTCTTGGCACTTG(+CAGTGT)GTACCCATGATCAACCTTCCCATCCCTCCCT NGG 10 8 17 8 24 0 100 5 10
    240605205328_fmmz AGGAGAGGGAGGGATGGGAAGGTTGATCATGG(+CAGTGT)GTACCAAGTGCCAAGAGCAAACAGCTTTGGT NGG 10 8 17 8 24 0 100 5 10