Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605205451_xhur TGTGACTGATTATATGGTCAATTTTGGAGAAG(+CAGTGT)GTACCATGAGATGCTGAGAAGAAGGTATATT NGG 10 8 17 8 24 0 100 5 10
    240605205449_qeao AAAAGAATATACCTTCTTCTCAGCATCTCATG(+CAGTGT)GTACCTTCTCCAAAATTGACCATATAATCAG NGG 10 8 17 8 24 0 100 5 10
    240605205448_t6kz GCCTTAGTTGCTATTTTACAGTACTGTTAAAG(+CAGTGT)GATCCATTGGCATGGGATGGGAAGCAGCAGG NGG 10 8 17 8 24 0 100 5 10
    240605205446_xwr9 TGGGCCCTGCTGCTTCCCATCCCATGCCAATG(+CAGTGT)GATCCTTTAACAGTACTGTAAAATAGCAACT NGG 10 8 17 8 24 0 100 5 10
    240605205443_htsg TCGACTTGCCCAGAAAGCATATGCCACAACAG(+CAGTGT)GATCCTTCTGCACACGTTTATTGGGAGAGCT NGG 10 8 17 8 24 0 100 5 10
    240605205441_y0qt AATCAAGCTCTCCCAATAAACGTGTGCAGAAG(+CAGTGT)GATCCTGTTGTGGCATATGCTTTCTGGGCAA NGG 10 8 17 8 24 0 100 5 10
    240605205439_1wsd AGCACTCTTGGGAGACCTGCTCACTGTTGGAG(+CAGTGT)GTACCTGGGTATGGAGAGCTGTGGCATAGGG NGG 10 8 17 8 24 0 100 5 10
    240605205436_hfsn GCTGACCCTATGCCACAGCTCTCCATACCCAG(+CAGTGT)GTACCTCCAACAGTGAGCAGGTCTCCCAAGA NGG 10 8 17 8 24 0 100 5 10
    240605205434_hecb GCCAACTGCGGCTACATGTCCCAGGGTGGGTG(+CAGTGT)GTACCAAAGTGGGGAGAAGAGGAGGGTGTAA NGG 10 8 17 8 24 0 100 5 10
    240605205433_5706 GCCCATTACACCCTCCTCTTCTCCCCACTTTG(+CAGTGT)GTACCACCCACCCTGGGACATGTAGCCGCAG NGG 10 8 17 8 24 0 100 5 10