Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605205558_kgxb GTGTGGCCAGTGCCAAGACCACCGTCTCTTGG(+CAGTGT)GATCCTCTGTCTTTATCTTTTGTTCGTCTGT NGG 10 8 17 8 24 0 100 5 10
    240605205557_9ia2 ATGAAACAGACGAACAAAAGATAAAGACAGAG(+CAGTGT)GATCCCAAGAGACGGTGGTCTTGGCACTGGC NGG 10 8 17 8 24 0 100 5 10
    240605205555_n5lp CCCGAGCCCTTCACGGGCATCCGAGGGAAAGG(+CAGTGT)GTACCAGAAGCATTCCCTCTTAATCATTGCA NGG 10 8 17 8 24 0 100 5 10
    240605205554_b34s ATGTCTGCAATGATTAAGAGGGAATGCTTCTG(+CAGTGT)GTACCCTTTCCCTCGGATGCCCGTGAAGGGC NGG 10 8 17 8 24 0 100 5 10
    240605205553_cj31 GAGAACAGAACAGAACTTCAGAGATCAAAGTG(+CAGTGT)GATCCGAGGGTAGATGTACCTTAGCCCAGAG NGG 10 8 17 8 24 0 100 5 10
    240605205550_r5yh TTTTACTCTGGGCTAAGGTACATCTACCCTCG(+CAGTGT)GATCCACTTTGATCTCTGAAGTTCTGTTCTG NGG 10 8 17 8 24 0 100 5 10
    240605205549_abxj AATGACCAAGTCAAAGTGTACAGGGTAAGTTG(+CAGTGT)GTACCTCAGCCTGGGCCAAGGAGAGATTCAA NGG 10 8 17 8 24 0 100 5 10
    240605205548_k5sj ATTACTTGAATCTCTCCTTGGCCCAGGCTGAG(+CAGTGT)GTACCAACTTACCCTGTACACTTTGACTTGG NGG 10 8 17 8 24 0 100 5 10
    240605205546_8ehe GAGCTTGATTACTTGGGGCTTGTTGTGGGAAG(+CAGTGT)GTACCATTGGGGATTGACCTCAGAGCCCCTA NGG 10 8 17 8 24 0 100 5 10
    240605205544_xtx8 TACTCTAGGGGCTCTGAGGTCAATCCCCAATG(+CAGTGT)GTACCTTCCCACAACAAGCCCCAAGTAATCA NGG 10 8 17 8 24 0 100 5 10