Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605205415_ldq4 CAGTTACTAAGGCACATGACCGTGGTGAGGAG(+CAGTGT)GATCCTCAGGACCTGCCGGAGCTGCCTGCAG NGG 10 8 17 8 24 0 100 5 10
    240605205413_gnbu GGTGGCTGCAGGCAGCTCCGGCAGGTCCTGAG(+CAGTGT)GATCCTCCTCACCACGGTCATGTGCCTTAGT NGG 10 8 17 8 24 0 100 5 10
    240605205411_7ox7 CTGTGTTTAGGTGTCACGTGTGAGATAATGTG(+CAGTGT)GTACCAGGAAAGGAGCTATGTACTGAGGCTT NGG 10 8 17 8 24 0 100 5 10
    240605205409_c7nb GAAACAAGCCTCAGTACATAGCTCCTTTCCTG(+CAGTGT)GTACCACATTATCTCACACGTGACACCTAAA NGG 10 8 17 8 24 0 100 5 10
    240605205408_5yam CAGCACAGTAGAGTCACCGAGGGGTATGGTAG(+CAGTGT)GTACCAGAGGAACTATTCAGTCCTCATGGAA NGG 10 8 17 8 24 0 100 5 10
    240605205406_xwes GTCTGTTCCATGAGGACTGAATAGTTCCTCTG(+CAGTGT)GTACCTACCATACCCCTCGGTGACTCTACTG NGG 10 8 17 8 24 0 100 5 10
    240605205404_yjki CTCTCAATCGGGGCTGGAAAAGAACAAAGTGG(+CAGTGT)GATCCCACACTTCTCCTGCACCCTCCAGCAG NGG 10 8 17 8 24 0 100 5 10
    240605205403_vx0e GCAACCTGCTGGAGGGTGCAGGAGAAGTGTGG(+CAGTGT)GATCCCACTTTGTTCTTTTCCAGCCCCGATT NGG 10 8 17 8 24 0 100 5 10
    240605205401_s6he GCCATCCATGGAATGAGTTTATGTGTACAAAG(+CAGTGT)GATCCAGAAAAGGGGACATTTTAAAAAAATC NGG 10 8 17 8 24 0 100 5 10
    240605205400_iokw ACAGTGATTTTTTTAAAATGTCCCCTTTTCTG(+CAGTGT)GATCCTTTGTACACATAAACTCATTCCATGG NGG 10 8 17 8 24 0 100 5 10