Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605203105_5sz1 TTTTTGTGAGGGAGTTGATCCTGGGCAGTTAG(+CAGTGT)GTACCCTGGAAGGAGGGGAGGGAACCTCGAT NGG 10 8 17 8 24 0 100 5 10
    240605203103_w2zd GCTTCATCGAGGTTCCCTCCCCTCCTTCCAGG(+CAGTGT)GTACCTAACTGCCCAGGATCAACTCCCTCAC NGG 10 8 17 8 24 0 100 5 10
    240605203101_27r3 ACCGGACCTTGTGGCCAAGTAGGATTCTGAGG(+CAGTGT)GATCCAGACGAGAAGCCCAAAGAGTTCAGAT NGG 10 8 17 8 24 0 100 5 10
    240605203059_u1am CCGGCATCTGAACTCTTTGGGCTTCTCGTCTG(+CAGTGT)GATCCCTCAGAATCCTACTTGGCCACAAGGT NGG 10 8 17 8 24 0 100 5 10
    240605203058_gl7i AGTCCTTAGCAGCCCCCACCACCCACGCATCG(+CAGTGT)GATCCCATTCTCAACCAGGACCCCCGCACCT NGG 10 8 17 8 24 0 100 5 10
    240605203056_t4gu TGGACAGGTGCGGGGGTCCTGGTTGAGAATGG(+CAGTGT)GATCCGATGCGTGGGTGGTGGGGGCTGCTAA NGG 10 8 17 8 24 0 100 5 10
    240605203054_9u1g GTCATCTTGTCAGTTCCTAACTGGAACCTTGG(+CAGTGT)GATCCTTCTTCTTCCTCTCTTTCTTTCTTTC NGG 10 8 17 8 24 0 100 5 10
    240605203053_vl5c AGAAAGAAAGAAAGAAAGAGAGGAAGAAGAAG(+CAGTGT)GATCCCAAGGTTCCAGTTAGGAACTGACAAG NGG 10 8 17 8 24 0 100 5 10
    240605203051_kv4n CTGATACTTGATGTTCCAGCCCAGCACCTCTG(+CAGTGT)GATCCTTGAAGTACCCATGATGGGTAATAGG NGG 10 8 17 8 24 0 100 5 10
    240605203050_v0go AAAGGCCTATTACCCATCATGGGTACTTCAAG(+CAGTGT)GATCCAGAGGTGCTGGGCTGGAACATCAAGT NGG 10 8 17 8 24 0 100 5 10