Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605203024_n24o GCTTCCTGATAAAACTGCCACAGACCACAGAG(+CAGTGT)GATCCTTGACAAAGCATTCTGGAAAATAGAG NGG 10 8 17 8 24 0 100 5 10
    240605203022_hj1n AGGACAGAAGAGGAGAAAGGGAAGGCCTGAGG(+CAGTGT)GTACCTCAGCCGCCAGCTACCTTCACTTTGA NGG 10 8 17 8 24 0 100 5 10
    240605203021_p2r0 AGACTTCAAAGTGAAGGTAGCTGGCGGCTGAG(+CAGTGT)GTACCCTCAGGCCTTCCCTTTCTCCTCTTCT NGG 10 8 17 8 24 0 100 5 10
    240605203019_xad5 GTAAACAGCAAGAGGCTTTATTGGGAACACGG(+CAGTGT)GTACCCGGGCGACTCAGTCTATCGGAGGACT NGG 10 8 17 8 24 0 100 5 10
    240605203016_t2r9 GCGCCAGTCCTCCGATAGACTGAGTCGCCCGG(+CAGTGT)GTACCCGTGTTCCCAATAAAGCCTCTTGCTG NGG 10 8 17 8 24 0 100 5 10
    240605203014_6a9f TTATGCAGATGCTCTTGCTGTGTTCCTCAAAG(+CAGTGT)GATCCAAGCCATAAAATTCTTTATGCATGTC NGG 10 8 17 8 24 0 100 5 10
    240605203013_hk1q ACTGTGACATGCATAAAGAATTTTATGGCTTG(+CAGTGT)GATCCTTTGAGGAACACAGCAAGAGCATCTG NGG 10 8 17 8 24 0 100 5 10
    240605203011_b73u AATGAAGTTCACTGTGTTTATAAACTGAAAAG(+CAGTGT)GTACCGGGTCTTACAGCATTTTATATATCAC NGG 10 8 17 8 24 0 100 5 10
    240605203009_6mer AAGGTGTGATATATAAAATGCTGTAAGACCCG(+CAGTGT)GTACCTTTTCAGTTTATAAACACAGTGAACT NGG 10 8 17 8 24 0 100 5 10
    240605203007_lx9u GGTTCGGTGCCTCCCCTTCCCCAGCAGCCAAG(+CAGTGT)GATCCTGGGGCCCTGTACAATATAGATGCTC NGG 10 8 17 8 24 0 100 5 10