Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605202918_x5p5 GCTGACCACATGGGGGTATTTGAACCTATGTG(+CAGTGT)GATCCTGGCTTATAATGTGTAAGAGTATTTC NGG 10 8 17 8 24 0 100 5 10
    240605202916_bk87 TCGAATGCAATACATTTCTGAGGCTCTCCATG(+CAGTGT)GTACCTACTTCCTCTCCTGACTCCATCCTTG NGG 10 8 17 8 24 0 100 5 10
    240605202914_9019 GTGATCAAGGATGGAGTCAGGAGAGGAAGTAG(+CAGTGT)GTACCATGGAGAGCCTCAGAAATGTATTGCA NGG 10 8 17 8 24 0 100 5 10
    240605202912_lmxu CCCAGTTCTCCCTCCACAGCGAGCTATCATGG(+CAGTGT)GTACCCCACACACCAGAAAAGCAAGACTCTG NGG 10 8 17 8 24 0 100 5 10
    240605202910_1rc2 TAAATCAGAGTCTTGCTTTTCTGGTGTGTGGG(+CAGTGT)GTACCCATGATAGCTCGCTGTGGAGGGAGAA NGG 10 8 17 8 24 0 100 5 10
    240605202909_mo3e AACAGAATACTAGAGAGAGAAGAGAATCTCAG(+CAGTGT)GTACCGAAGATAACATAGAAAACATCCAGTG NGG 10 8 17 8 24 0 100 5 10
    240605202908_ncoe TTTTCCACTGGATGTTTTCTATGTTATCTTCG(+CAGTGT)GTACCTGAGATTCTCTTCTCTCTCTAGTATT NGG 10 8 17 8 24 0 100 5 10
    240605202905_kmtf ATCGAGAAGCCATCCTGGGATGAAGCCTCTGG(+CAGTGT)GATCCATGTCTCTCAGCTCTCACCCACTGCA NGG 10 8 17 8 24 0 100 5 10
    240605202904_2hj2 ATGGCTGCAGTGGGTGAGAGCTGAGAGACATG(+CAGTGT)GATCCCAGAGGCTTCATCCCAGGATGGCTTC NGG 10 8 17 8 24 0 100 5 10
    240605202903_lkoi TAAATACCTGAGCCCCCTTTTTGTTCTTCACG(+CAGTGT)GATCCTTGGTCTTAATGTTGAAACACCTAAT NGG 10 8 17 8 24 0 100 5 10