Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605202742_w5k1 AGCCCCAGCTTCCTCCTGAGCACAGGTTTGGG(+CAGTGT)GATCCAGGCGTTGTATTGACCCTTATGGGAG NGG 10 8 17 8 24 0 100 5 10
    240605202740_tvj8 CCCTGATGGAGGAGGCCAGGGCTCAGAGCAGG(+CAGTGT)GTACCCACTGTGGATCTTTGTCTGTGAACCG NGG 10 8 17 8 24 0 100 5 10
    240605202738_bxf5 GTTGTCGGTTCACAGACAAAGATCCACAGTGG(+CAGTGT)GTACCCTGCTCTGAGCCCTGGCCTCCTCCAT NGG 10 8 17 8 24 0 100 5 10
    240605202735_gv47 TCTCGAGACTGACTCAGGGTCCAGAGCCACCG(+CAGTGT)GATCCATCCCCAAGTGGAGATGATGTCGGAG NGG 10 8 17 8 24 0 100 5 10
    240605202734_31bj AACCTCTCCGACATCATCTCCACTTGGGGATG(+CAGTGT)GATCCGGTGGCTCTGGACCCTGAGTCAGTCT NGG 10 8 17 8 24 0 100 5 10
    240605202731_tscf CAAGTTCGAGCCAGATATGGAGATCAGGAAGG(+CAGTGT)GTACCTTCTGAGGCTTGTAGGCATGGAGGAG NGG 10 8 17 8 24 0 100 5 10
    240605202730_xgg5 GCTTGCTCCTCCATGCCTACAAGCCTCAGAAG(+CAGTGT)GTACCCTTCCTGATCTCCATATCTGGCTCGA NGG 10 8 17 8 24 0 100 5 10
    240605202728_4y33 GAAGCCTGTTTCCTCCTATGAAAAGTACAATG(+CAGTGT)GTACCTACTTTACAGAGCTGTGTTAATGCAT NGG 10 8 17 8 24 0 100 5 10
    240605202726_xn8m ATTATATGCATTAACACAGCTCTGTAAAGTAG(+CAGTGT)GTACCATTGTACTTTTCATAGGAGGAAACAG NGG 10 8 17 8 24 0 100 5 10
    240605202725_8ws5 CCTTGCACAAAATGGAGTTTCTCATCCTGCTG(+CAGTGT)GATCCTTTAATGTGTCAGTCCCTGGAAGACC NGG 10 8 17 8 24 0 100 5 10