Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605202644_ioxb TCCACCCAGGTGAATCCTGGCACTGCCTGCCG(+CAGTGT)GATCCCCGTCTGCCGGATGACATGCTCTGCC NGG 10 8 17 8 24 0 100 5 10
    240605202643_8b6u GTACCCACAGAGAAGGCCAGAAGAAAGCACTG(+CAGTGT)GATCCCGGGAACTGAAGTCATAGATGGCTGT NGG 10 8 17 8 24 0 100 5 10
    240605202639_3ex6 GGTTCACAGCCATCTATGACTTCAGTTCCCGG(+CAGTGT)GATCCAGTGCTTTCTTCTGGCCTTCTCTGTG NGG 10 8 17 8 24 0 100 5 10
    240605202638_7hlz AGAAACCTACTTGCACATTCTCAGTACACTTG(+CAGTGT)GATCCAAAATGTCTACACTTGATGCTCCGGC NGG 10 8 17 8 24 0 100 5 10
    240605202637_q23l CTTGGGCCGGAGCATCAAGTGTAGACATTTTG(+CAGTGT)GATCCAAGTGTACTGAGAATGTGCAAGTAGG NGG 10 8 17 8 24 0 100 5 10
    240605202635_r9ps GTTCACTTCCTCAATAAGGGCCTGATTTGGGG(+CAGTGT)GTACCAACCTTCTCTCTCCCATTGACCAGTG NGG 10 8 17 8 24 0 100 5 10
    240605202633_tc4q CAGAGCACTGGTCAATGGGAGAGAGAAGGTTG(+CAGTGT)GTACCCCCAAATCAGGCCCTTATTGAGGAAG NGG 10 8 17 8 24 0 100 5 10
    240605202632_mkfn CCAAGCATGGGGCCCAAGGGAGGAGGGATCAG(+CAGTGT)GATCCTAATTTTGCTCAGAGGAAGTGAACTT NGG 10 8 17 8 24 0 100 5 10
    240605202631_f9fu ATTGGAAGTTCACTTCCTCTGAGCAAAATTAG(+CAGTGT)GATCCTGATCCCTCCTCCCTTGGGCCCCATG NGG 10 8 17 8 24 0 100 5 10
    240605202630_yyxv AGGCAGATCCTGTGGTTCTGGAAGATACTCTG(+CAGTGT)GATCCAGAGTCCTTGGTAGAGAAGCCTCCAG NGG 10 8 17 8 24 0 100 5 10