Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605202251_cd5s CCATAGCCCTTTATATGCCAACAAGTCTGTAG(+CAGTGT)GATCCTTGAGAGCCTGGGAATCATACTTCTT NGG 10 8 17 8 24 0 100 5 10
    240605202250_eo9x CACTGCCCTCAGAACTCAGGGTTTGGGGCAAG(+CAGTGT)GATCCAGGCACGATAGGGCCCAACGGCTCGC NGG 10 8 17 8 24 0 100 5 10
    240605202247_divf CAGCCGCGAGCCGTTGGGCCCTATCGTGCCTG(+CAGTGT)GATCCTTGCCCCAAACCCTGAGTTCTGAGGG NGG 10 8 17 8 24 0 100 5 10
    240605202246_p0fw TCCAGCAAACATTTATTGATAACCCAAGTAAG(+CAGTGT)GTACCCTGCAGATGTAGAAAAAAGCATCATG NGG 10 8 17 8 24 0 100 5 10
    240605202244_u8to CCATCCATGATGCTTTTTTCTACATCTGCAGG(+CAGTGT)GTACCTTACTTGGGTTATCAATAAATGTTTG NGG 10 8 17 8 24 0 100 5 10
    240605202243_lgxt CTGTAAATATAATTTATTGAGGCCTGTAGTTG(+CAGTGT)GTACCAGGGCCTTTATTCTGTTTGCATTTCC NGG 10 8 17 8 24 0 100 5 10
    240605202240_8idj AAGTCGGAAATGCAAACAGAATAAAGGCCCTG(+CAGTGT)GTACCAACTACAGGCCTCAATAAATTATATT NGG 10 8 17 8 24 0 100 5 10
    240605202239_u1v2 TGCTTGTTGGTTTCTCTCCAGTTCTAGAACTG(+CAGTGT)GATCCTTTAGTTTTCCTGGGCTTAGCCCTGA NGG 10 8 17 8 24 0 100 5 10
    240605202237_tjh9 TATTCTCAGGGCTAAGCCCAGGAAAACTAAAG(+CAGTGT)GATCCAGTTCTAGAACTGGAGAGAAACCAAC NGG 10 8 17 8 24 0 100 5 10
    240605202235_x250 CTAAGTGGTTCACAACCACCTAGAATCCAGAG(+CAGTGT)GATCCGATATCCTCTTCTGGTATCCGTGGGC NGG 10 8 17 8 24 0 100 5 10