Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605202405_3wi4 AAACCACCTGCAAGCTCTGTCATAGAGCCCAG(+CAGTGT)GATCCCTGAGGACATTCTGTCCTAAGCTCCC NGG 10 8 17 8 24 0 100 5 10
    240605202404_djvr GATGCATATGTGATGACTTTCTTAGCAGTTGG(+CAGTGT)GATCCACTCATATGTTTCAAAACTTTACCTA NGG 10 8 17 8 24 0 100 5 10
    240605202402_j6ek ACTCCTAGGTAAAGTTTTGAAACATATGAGTG(+CAGTGT)GATCCCAACTGCTAAGAAAGTCATCACATAT NGG 10 8 17 8 24 0 100 5 10
    240605202400_a9kb AAATAAGACCAGGACCACAGGGAATCTACCAG(+CAGTGT)GATCCTTCCCACGCCCTTCCTGAAGCATCAA NGG 10 8 17 8 24 0 100 5 10
    240605202358_d3ea GATCGTTGATGCTTCAGGAAGGGCGTGGGAAG(+CAGTGT)GATCCTGGTAGATTCCCTGTGGTCCTGGTCT NGG 10 8 17 8 24 0 100 5 10
    240605202357_sku8 CCTCGACATGGTCTCCTGGGGTGGTTTCTAAG(+CAGTGT)GTACCTTCCTGTTTAATACTACATAACCTCG NGG 10 8 17 8 24 0 100 5 10
    240605202355_9uas CTTTCCGAGGTTATGTAGTATTAAACAGGAAG(+CAGTGT)GTACCTTAGAAACCACCCCAGGAGACCATGT NGG 10 8 17 8 24 0 100 5 10
    240605202352_niqt AAATAGGCAGTCACCCGAAAAGACTTTTCCTG(+CAGTGT)GATCCGTTTGCCTCAGCTATTTGTGGGAGGC NGG 10 8 17 8 24 0 100 5 10
    240605202349_htq2 ACCAAGCCTCCCACAAATAGCTGAGGCAAACG(+CAGTGT)GATCCAGGAAAAGTCTTTTCGGGTGACTGCC NGG 10 8 17 8 24 0 100 5 10
    240605202348_gwrl ATCGGTAGGCACGCTGCTCCTCTTGCTGCAGG(+CAGTGT)GTACCCCAGGGGAGGTGTTCTTGGCTACCAG NGG 10 8 17 8 24 0 100 5 10