Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605202500_t0cl CTTCATTATTCAAACAGGGCTCGTTAAGTTTG(+CAGTGT)GATCCAGCTGTGGACCATCCCTGAGATTCAA NGG 10 8 17 8 24 0 100 5 10
    240605202457_nwpf CAAATCACTCCTGTGCATAGCACTTTCCCTGG(+CAGTGT)GATCCACAATTCAGCCCTGGGTCCCCACCCC NGG 10 8 17 8 24 0 100 5 10
    240605202456_5vj8 GGTAAGGGGTGGGGACCCAGGGCTGAATTGTG(+CAGTGT)GATCCCAGGGAAAGTGCTATGCACAGGAGTG NGG 10 8 17 8 24 0 100 5 10
    240605202454_qa9v ATTATCCAACTCCCACCCCAGGATCTCCACTG(+CAGTGT)GTACCCAGATGCCCTTAGACCCTTTCATCTG NGG 10 8 17 8 24 0 100 5 10
    240605202452_hqbi GCTTCCAGATGAAAGGGTCTAAGGGCATCTGG(+CAGTGT)GTACCAGTGGAGATCCTGGGGTGGGAGTTGG NGG 10 8 17 8 24 0 100 5 10
    240605202451_jbkz ACCTCAAGGCTTGGGTGACACACACAAATGTG(+CAGTGT)GTACCTCAGCCAGGGCCAGGACCTGCTAGGG NGG 10 8 17 8 24 0 100 5 10
    240605202450_sb3q TGGACCCCTAGCAGGTCCTGGCCCTGGCTGAG(+CAGTGT)GTACCACATTTGTGTGTGTCACCCAAGCCTT NGG 10 8 17 8 24 0 100 5 10
    240605202448_gqot TTCTACAGGCTTAGGGGCCTTTCCAGGGATGG(+CAGTGT)GTACCACCAAGGACTAACCACTTTACTTTGT NGG 10 8 17 8 24 0 100 5 10
    240605202446_81ny GGGGAACAAAGTAAAGTGGTTAGTCCTTGGTG(+CAGTGT)GTACCCATCCCTGGAAAGGCCCCTAAGCCTG NGG 10 8 17 8 24 0 100 5 10
    240605202445_c510 TCTAATAGGTCATCCTGTGGCTTCCAGAGTAG(+CAGTGT)GTACCGCCAGCCCAGCTCAAGTTCTCTGCTT NGG 10 8 17 8 24 0 100 5 10