Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605202516_q4q9 TCTGCCTCTCCCTGCTGGAGCTCAGGGGCCTG(+CAGTGT)GTACCTGGGCTCTGCACTTTGCCACCCCCAG NGG 10 8 17 8 24 0 100 5 10
    240605202513_1eo5 CTTCCTCCAAGGACAGGCCTGTGAGTTCCGGG(+CAGTGT)GTACCTGTTACCTATTCCAGACATCTGAGTA NGG 10 8 17 8 24 0 100 5 10
    240605202512_ycql CCAGGTACTCAGATGTCTGGAATAGGTAACAG(+CAGTGT)GTACCCCGGAACTCACAGGCCTGTCCTTGGA NGG 10 8 17 8 24 0 100 5 10
    240605202510_w7pn TACCCTGAGCCAGGCATTTTAACACTACAGTG(+CAGTGT)GTACCCACACAAGGCACATGGGATGATTAGC NGG 10 8 17 8 24 0 100 5 10
    240605202508_9gy8 AAGGTGCTAATCATCCCATGTGCCTTGTGTGG(+CAGTGT)GTACCACTGTAGTGTTAAAATGCCTGGCTCA NGG 10 8 17 8 24 0 100 5 10
    240605202507_vvgn AGCTGCTGTGAAGAGGGCAAGGGTTAAAAATG(+CAGTGT)GATCCACTGCTGCTTTCCTTTTTCCTTCTTT NGG 10 8 17 8 24 0 100 5 10
    240605202505_iahw AATAAAAAGAAGGAAAAAGGAAAGCAGCAGTG(+CAGTGT)GATCCATTTTTAACCCTTGCCCTCTTCACAG NGG 10 8 17 8 24 0 100 5 10
    240605202504_ej5c CTAGACATCATTCATAGAAACAATAAAGAATG(+CAGTGT)GTACCAATGTAGAGGAGCAAAGCTGCTGGGT NGG 10 8 17 8 24 0 100 5 10
    240605202503_x1bk AGGAAACCCAGCAGCTTTGCTCCTCTACATTG(+CAGTGT)GTACCATTCTTTATTGTTTCTATGAATGATG NGG 10 8 17 8 24 0 100 5 10
    240605202501_6ulu AAACATTGAATCTCAGGGATGGTCCACAGCTG(+CAGTGT)GATCCAAACTTAACGAGCCCTGTTTGAATAA NGG 10 8 17 8 24 0 100 5 10