Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605202424_bn3r GAGCATATAACCAAAGTGGTTTGGCTTCTGAG(+CAGTGT)GATCCTAGAAAAAGGTAGTTCTAATTTCTCT NGG 10 8 17 8 24 0 100 5 10
    240605202422_eo0i TGGTGAGTAAGCCAGCGTCGGCTCAGAGCCTG(+CAGTGT)GATCCCAGTTACTTCCACATCTGCGGGGCCA NGG 10 8 17 8 24 0 100 5 10
    240605202420_dym5 AACTGTGGCCCCGCAGATGTGGAAGTAACTGG(+CAGTGT)GATCCAGGCTCTGAGCCGACGCTGGCTTACT NGG 10 8 17 8 24 0 100 5 10
    240605202418_awsq GTTATGGCCCAGCTGCTGCCACCTTGTCCTTG(+CAGTGT)GTACCATAAGATATCATGGAACGCCCAGTTC NGG 10 8 17 8 24 0 100 5 10
    240605202417_k1op GTGTGGAACTGGGCGTTCCATGATATCTTATG(+CAGTGT)GTACCAAGGACAAGGTGGCAGCAGCTGGGCC NGG 10 8 17 8 24 0 100 5 10
    240605202415_6e0o AATTTTACCATCAATACAGGCTCAGTGATAGG(+CAGTGT)GTACCCTTTTCCAGGAACTGGGTTTAATTGC NGG 10 8 17 8 24 0 100 5 10
    240605202413_czl6 TGCTGGCAATTAAACCCAGTTCCTGGAAAAGG(+CAGTGT)GTACCCTATCACTGAGCCTGTATTGATGGTA NGG 10 8 17 8 24 0 100 5 10
    240605202411_c2oi TGATAGAAAAGTCAGAAATTCCATCAGTGTGG(+CAGTGT)GTACCAGGAAATGTGGTTGGACCAGCCCTGG NGG 10 8 17 8 24 0 100 5 10
    240605202409_g73t GGCATCCAGGGCTGGTCCAACCACATTTCCTG(+CAGTGT)GTACCCACACTGATGGAATTTCTGACTTTTC NGG 10 8 17 8 24 0 100 5 10
    240605202407_squ1 GTGTTGGGAGCTTAGGACAGAATGTCCTCAGG(+CAGTGT)GATCCTGGGCTCTATGACAGAGCTTGCAGGT NGG 10 8 17 8 24 0 100 5 10