Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605202441_vt6d CCGTTAAGCAGAGAACTTGAGCTGGGCTGGCG(+CAGTGT)GTACCTACTCTGGAAGCCACAGGATGACCTA NGG 10 8 17 8 24 0 100 5 10
    240605202439_4pvt TTCTTTTGTTTCTTATAAGACGAATATTGAGG(+CAGTGT)GATCCTTGTATTTCTTTCTTTTGTATTTTTT NGG 10 8 17 8 24 0 100 5 10
    240605202438_h3ng CAAGAAAAAAATACAAAAGAAAGAAATACAAG(+CAGTGT)GATCCCTCAATATTCGTCTTATAAGAAACAA NGG 10 8 17 8 24 0 100 5 10
    240605202436_p27f TAGGGAGAGGCACTGAGAAGATGACAGGGCAG(+CAGTGT)GATCCAAGCCCTTCAACCAGAGCAAACTGGG NGG 10 8 17 8 24 0 100 5 10
    240605202435_k34h TTTCTCCCAGTTTGCTCTGGTTGAAGGGCTTG(+CAGTGT)GATCCTGCCCTGTCATCTTCTCAGTGCCTCT NGG 10 8 17 8 24 0 100 5 10
    240605202433_3kc3 GGAAGCCTTCCATGAAACAAATCTTGCAGCAG(+CAGTGT)GATCCCACCCAAGCTGGGTGGAGTTGAAGGA NGG 10 8 17 8 24 0 100 5 10
    240605202431_7xbk ACAGGTCCTTCAACTCCACCCAGCTTGGGTGG(+CAGTGT)GATCCTGCTGCAAGATTTGTTTCATGGAAGG NGG 10 8 17 8 24 0 100 5 10
    240605202429_4b1x GTTGAACTCAAGTACAACAGACCATACAATTG(+CAGTGT)GTACCATTCAAAATAATGACACTCCTTTCTT NGG 10 8 17 8 24 0 100 5 10
    240605202428_s51g AGGCAAAGAAAGGAGTGTCATTATTTTGAATG(+CAGTGT)GTACCAATTGTATGGTCTGTTGTACTTGAGT NGG 10 8 17 8 24 0 100 5 10
    240605202426_xcjd CTGGCAGAGAAATTAGAACTACCTTTTTCTAG(+CAGTGT)GATCCTCAGAAGCCAAACCACTTTGGTTATA NGG 10 8 17 8 24 0 100 5 10