Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605193636_hx39 GTGGCTGCGTCACACAGAGATGGAAGCAAGTG(+CAGTGT)GATCCTCTGTATGCTTCTGTCTCCAGACGAG NGG 10 8 17 8 24 0 100 5 10
    240605193634_fz41 CAAAGCTCGTCTGGAGACAGAAGCATACAGAG(+CAGTGT)GATCCACTTGCTTCCATCTCTGTGTGACGCA NGG 10 8 17 8 24 0 100 5 10
    240605193633_29v5 CATGTGCCCACAGAAGCCAGAAGAGGGCGTCG(+CAGTGT)GATCCCTTGGAACTGGAATGGCTGAGTCATC NGG 10 8 17 8 24 0 100 5 10
    240605193630_yq2s GGAGAGATGACTCAGCCATTCCAGTTCCAAGG(+CAGTGT)GATCCGACGCCCTCTTCTGGCTTCTGTGGGC NGG 10 8 17 8 24 0 100 5 10
    240605193629_cvwz GTATTGAGATATGTAAAGACACCTGCAGACGG(+CAGTGT)GTACCTAGGGAGAAGACACACACATACACAC NGG 10 8 17 8 24 0 100 5 10
    240605193626_zhxl AGAATGTGTGTATGTGTGTGTCTTCTCCCTAG(+CAGTGT)GTACCCGTCTGCAGGTGTCTTTACATATCTC NGG 10 8 17 8 24 0 100 5 10
    240605193624_8mc7 GAGAGAAAAACAACTGGTCCAGAAATTCAGTG(+CAGTGT)GATCCATGACTTACGTGTCACAGAAGAATGA NGG 10 8 17 8 24 0 100 5 10
    240605193623_ebxs AACAGTCATTCTTCTGTGACACGTAAGTCATG(+CAGTGT)GATCCACTGAATTTCTGGACCAGTTGTTTTT NGG 10 8 17 8 24 0 100 5 10
    240605193622_o1vv CAACTTAACAAACTTGGCAAATGGAGAGTTAG(+CAGTGT)GATCCAGAGGGGGGGGGGGAAACACAACTTC NGG 10 8 17 8 24 0 100 5 10
    240605193620_w99j GCCCTGAAGTTGTGTTTCCCCCCCCCCCTCTG(+CAGTGT)GATCCTAACTCTCCATTTGCCAAGTTTGTTA NGG 10 8 17 8 24 0 100 5 10