Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605193725_m1ux CGTACAAAGTGGGCGGTGCTGAGGGGCTTGGG(+CAGTGT)GATCCTTGCTGCAAAGCTTTGCAGAATGTAA NGG 10 8 17 8 24 0 100 5 10
    240605193723_21x4 GCCTCTTACATTCTGCAAAGCTTTGCAGCAAG(+CAGTGT)GATCCCCAAGCCCCTCAGCACCGCCCACTTT NGG 10 8 17 8 24 0 100 5 10
    240605193722_q825 CCCTTCAGACTGAGTAAACTTTTCAATATATG(+CAGTGT)GTACCCAGATGTTTTATTTATGAAGTTTTTA NGG 10 8 17 8 24 0 100 5 10
    240605193720_3q11 TAAAATAAAAACTTCATAAATAAAACATCTGG(+CAGTGT)GTACCATATATTGAAAAGTTTACTCAGTCTG NGG 10 8 17 8 24 0 100 5 10
    240605193718_iedo AAACAAACCAAACAAAACATAAAAAGCCTTTG(+CAGTGT)GTACCTTTCAATAACAAAAGAAAAATATATT NGG 10 8 17 8 24 0 100 5 10
    240605193717_kn2n ATCTAAATATATTTTTCTTTTGTTATTGAAAG(+CAGTGT)GTACCAAAGGCTTTTTATGTTTTGTTTGGTT NGG 10 8 17 8 24 0 100 5 10
    240605193715_mqr2 GAGGCAACTGCAGACGAATCAGAAAATAAGAG(+CAGTGT)GATCCACCACCACAGAACTGATTGGCTCAGA NGG 10 8 17 8 24 0 100 5 10
    240605193713_iof7 ATTGCTCTGAGCCAATCAGTTCTGTGGTGGTG(+CAGTGT)GATCCTCTTATTTTCTGATTCGTCTGCAGTT NGG 10 8 17 8 24 0 100 5 10
    240605193711_2o3h GTAAGAATAAGTTGATAGCTTCAAATCTACAG(+CAGTGT)GATCCCTTCAGCTGGCACTATCTCCCTAAAA NGG 10 8 17 8 24 0 100 5 10
    240605193710_r1sd AGACATTTTAGGGAGATAGTGCCAGCTGAAGG(+CAGTGT)GATCCTGTAGATTTGAAGCTATCAACTTATT NGG 10 8 17 8 24 0 100 5 10