Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605193815_ec4t TATTTGGCTGTAGCTCTGATCAACTTTCCCTG(+CAGTGT)GTACCCTCATCCTACCTTCATATACAGGTAG NGG 10 8 17 8 24 0 100 5 10
    240605193813_ftta TGGAGCTACCTGTATATGAAGGTAGGATGAGG(+CAGTGT)GTACCAGGGAAAGTTGATCAGAGCTACAGCC NGG 10 8 17 8 24 0 100 5 10
    240605193810_9xtx CATCCCAAAGTTGGCCTTTGTCCTTCCCCATG(+CAGTGT)GATCCCTAAAAACAAACAAACAAATAAAAAA NGG 10 8 17 8 24 0 100 5 10
    240605193809_e5pv TTTATTTTTTTATTTGTTTGTTTGTTTTTAGG(+CAGTGT)GATCCATGGGGAAGGACAAAGGCCAACTTTG NGG 10 8 17 8 24 0 100 5 10
    240605193807_ec6v AGGTTCCAGACTTCTCCCAGCCACCGTGGCAG(+CAGTGT)GTACCCCTCATGTCAGGGACTGCCTCGTGCT NGG 10 8 17 8 24 0 100 5 10
    240605193805_nu1x AAAGCAGCACGAGGCAGTCCCTGACATGAGGG(+CAGTGT)GTACCTGCCACGGTGGCTGGGAGAAGTCTGG NGG 10 8 17 8 24 0 100 5 10
    240605193804_8du5 TAGTTAGCGATGAGGAGGAAGACACTTGAATG(+CAGTGT)GATCCCCACCCTAGGATGCTGCTCTTCTTGC NGG 10 8 17 8 24 0 100 5 10
    240605193802_sqo2 GGAAGGCAAGAAGAGCAGCATCCTAGGGTGGG(+CAGTGT)GATCCATTCAAGTGTCTTCCTCCTCATCGCT NGG 10 8 17 8 24 0 100 5 10
    240605193800_g6rh TATTGATTCTCTTAGAACTTTGTCAGTGGATG(+CAGTGT)GTACCTTGCCTTTGCAGGTTGTATGTGACCT NGG 10 8 17 8 24 0 100 5 10
    240605193759_cky2 ATCTAAGGTCACATACAACCTGCAAAGGCAAG(+CAGTGT)GTACCATCCACTGACAAAGTTCTAAGAGAAT NGG 10 8 17 8 24 0 100 5 10