Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605193846_henl GGCTGAGTGGAAGTTTGGTTTTAGGAAGTAAG(+CAGTGT)GTACCATACGACATTAATCTTTCAGTGAGTA NGG 10 8 17 8 24 0 100 5 10
    240605193845_mgzt GGGTTTACTCACTGAAAGATTAATGTCGTATG(+CAGTGT)GTACCTTACTTCCTAAAACCAAACTTCCACT NGG 10 8 17 8 24 0 100 5 10
    240605193844_47jg GGCAGAGAGACTGTCCTCCATTTTAAGACTTG(+CAGTGT)GTACCACTTTGAGAGATGAGTCCAGGGTCCC NGG 10 8 17 8 24 0 100 5 10
    240605193842_afay GCTGTGGGACCCTGGACTCATCTCTCAAAGTG(+CAGTGT)GTACCAAGTCTTAAAATGGAGGACAGTCTCT NGG 10 8 17 8 24 0 100 5 10
    240605193841_dkd4 AATTTTCATTTGTGTAAACTCCAAAAAAAAAG(+CAGTGT)GATCCTAACAACTATTTTGGGTTTTATCATT NGG 10 8 17 8 24 0 100 5 10
    240605193839_x68c AAGTAAATGATAAAACCCAAAATAGTTGTTAG(+CAGTGT)GATCCTTTTTTTTTGGAGTTTACACAAATGA NGG 10 8 17 8 24 0 100 5 10
    240605193837_hz2r TAATATTTAATGATTGAAATTAACTGTTTGGG(+CAGTGT)GATCCTGATGCTGATGTGTGTATTACTAAAG NGG 10 8 17 8 24 0 100 5 10
    240605193835_e6l9 AAATTCTTTAGTAATACACACATCAGCATCAG(+CAGTGT)GATCCCCAAACAGTTAATTTCAATCATTAAA NGG 10 8 17 8 24 0 100 5 10
    240605193834_qky7 TTTGATGCCAACAAAGTTTTAGGGATACAATG(+CAGTGT)GTACCTTGCCAACATCGAGTGACTTTAGCTG NGG 10 8 17 8 24 0 100 5 10
    240605193833_gz6f TCTTCCAGCTAAAGTCACTCGATGTTGGCAAG(+CAGTGT)GTACCATTGTATCCCTAAAACTTTGTTGGCA NGG 10 8 17 8 24 0 100 5 10