Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605193903_lhw9 TATTTGTGTGTGGAGAGAGAAAGAGACTGAAG(+CAGTGT)GATCCCCTTCCCTTTTTTTCTTTGTGCTAGG NGG 10 8 17 8 24 0 100 5 10
    240605193901_iu67 GAGGTCCTAGCACAAAGAAAAAAAGGGAAGGG(+CAGTGT)GATCCTTCAGTCTCTTTCTCTCTCCACACAC NGG 10 8 17 8 24 0 100 5 10
    240605193900_e4rs AAGTAGGGTGAGGCCTTAAGCTTTCAAATCTG(+CAGTGT)GATCCCAGGGAAGCACTTCCTTTAACAAGGC NGG 10 8 17 8 24 0 100 5 10
    240605193856_k7om TGGTGGCCTTGTTAAAGGAAGTGCTTCCCTGG(+CAGTGT)GATCCAGATTTGAAAGCTTAAGGCCTCACCC NGG 10 8 17 8 24 0 100 5 10
    240605193855_horb GCCACATGGAAATCTTTAATTTATTTGTTGAG(+CAGTGT)GTACCATATAGCCAGGGTGCTAGGTGATGAG NGG 10 8 17 8 24 0 100 5 10
    240605193853_dwga CACCACTCATCACCTAGCACCCTGGCTATATG(+CAGTGT)GTACCTCAACAAATAAATTAAAGATTTCCAT NGG 10 8 17 8 24 0 100 5 10
    240605193852_u4td AACTACTGGTTTGCTGTGTAAATGGATGTCAG(+CAGTGT)GATCCAGCAAAACTACTGCTTTCTTCTCCAA NGG 10 8 17 8 24 0 100 5 10
    240605193850_afpw TGTCTTTGGAGAAGAAAGCAGTAGTTTTGCTG(+CAGTGT)GATCCTGACATCCATTTACACAGCAAACCAG NGG 10 8 17 8 24 0 100 5 10
    240605193849_es2d CCTACTAGGTTCTAACTGATGCAGAGTGAAGG(+CAGTGT)GTACCGAGAAAGTACATGTACATAAAGATTT NGG 10 8 17 8 24 0 100 5 10
    240605193847_4fby CTCTTAAATCTTTATGTACATGTACTTTCTCG(+CAGTGT)GTACCCTTCACTCTGCATCAGTTAGAACCTA NGG 10 8 17 8 24 0 100 5 10