Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605193757_p43c CAAACAGAGACAAGCTTCTCTGAATGTGGGTG(+CAGTGT)GTACCATTCCCATAGGCTTTGGCCAAGGAGA NGG 10 8 17 8 24 0 100 5 10
    240605193756_kzsd TCTACTCTCCTTGGCCAAAGCCTATGGGAATG(+CAGTGT)GTACCACCCACATTCAGAGAAGCTTGTCTCT NGG 10 8 17 8 24 0 100 5 10
    240605193755_1pcb GCTTGGAAGCTTGAGACCCAAATAGAAGTTTG(+CAGTGT)GATCCAATGACTGCAAACTGCTCCTTTTCAG NGG 10 8 17 8 24 0 100 5 10
    240605193754_wgzu TCTCTCTGAAAAGGAGCAGTTTGCAGTCATTG(+CAGTGT)GATCCAAACTTCTATTTGGGTCTCAAGCTTC NGG 10 8 17 8 24 0 100 5 10
    240605193752_ll1b GTGCTTATGAGGTCATGGAAACCTGGTCTCAG(+CAGTGT)GATCCATAAAAGATGATGTTTATAGTTAAGA NGG 10 8 17 8 24 0 100 5 10
    240605193751_twkt AAATATCTTAACTATAAACATCATCTTTTATG(+CAGTGT)GATCCTGAGACCAGGTTTCCATGACCTCATA NGG 10 8 17 8 24 0 100 5 10
    240605193748_s085 TGGTCTTTATTTAGAGTCCATAACCCCTCCAG(+CAGTGT)GTACCTCAATCCTTCCCATAAATCTTCCACA NGG 10 8 17 8 24 0 100 5 10
    240605193746_ayfm GATCTTGTGGAAGATTTATGGGAAGGATTGAG(+CAGTGT)GTACCTGGAGGGGTTATGGACTCTAAATAAA NGG 10 8 17 8 24 0 100 5 10
    240605193745_dc3l GGTTAGACAGGCCAGCCCAGCTAGGAGAGCAG(+CAGTGT)GATCCACAGGTAGACGGCAGAGTCATGGTAA NGG 10 8 17 8 24 0 100 5 10
    240605193743_g3gf GTGGCTTACCATGACTCTGCCGTCTACCTGTG(+CAGTGT)GATCCTGCTCTCCTAGCTGGGCTGGCCTGTC NGG 10 8 17 8 24 0 100 5 10